Glycated apolipoprotein A-I assay by combination of affinity chromatography and latex immunoagglutination

• Published in: Annals of clinical biochemistry Vol. 37 ( Pt 4); p. 498
• Main Authors: Shishino, K, Murase, M, Makino, H, Saheki, S
• Format: Journal Article
• Language: English
• Published: England 01.07.2000
• Subjects: Agglutination; Apolipoprotein A-I - blood; Apolipoprotein A-I - metabolism; Blood Glucose - metabolism; Boronic Acids - metabolism; Case-Control Studies; Chemistry, Clinical - methods; Chromatography, Affinity - methods; Chromatography, Gel; Diabetes Mellitus - blood; Dose-Response Relationship, Drug; Female; Humans; Immunoassay; Male; Sensitivity and Specificity; Sex Factors; Time Factors
• ISSN: 0004-5632
• DOI: 10.1177/000456320003700411

The degree of glycation of plasma apolipoprotein A-I was measured by a combination of gel filtration, boronate affinity chromatography and latex immunoagglutination. The plasma concentrations of apolipoprotein A-I determined by this combination method (y) correlated well with those determined by turbidimetric immunoassay (x) (y=1.12x + 1.9, r=0.964). The inter- and intra-assay coefficients of variation in the glycated apolipoprotein A-I assay were 4.1-5.0% and 4.0-4.4%, respectively. Interference from plasma glucose at concentrations up to 55.1 mmol/L was eliminated by gel filtration. Labile glycated apolipoprotein A-I did not interfere with the measurement of glycated apolipoprotein A-I. Reference values for glycated apolipoprotein A-I were determined to be 2.4-4.0% (n=140), with no significant difference between men and women. The mean concentration of plasma glycated apolipoprotein A-I in patients with uncontrolled diabetes mellitus (5.11%) was significantly higher than in normal subjects (3.12%, P<0.001). The method is simple, rapid and highly sensitive for determination of the glycation level of plasma apolipoprotein A-I.