Design for preparation of more active cross-linked enzyme aggregates of Burkholderia cepacia lipase using palm fiber residue

• Published in: Bioprocess and biosystems engineering Vol. 44; no. 1; pp. 57 - 66
• Main Authors: Alves, Nanda R., Pereira, Matheus M., Giordano, Raquel L. C., Tardioli, Paulo W., Lima, Álvaro S., Soares, Cleide M. F., Souza, Ranyere L.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 2021; Springer Nature B.V
• Subjects: Additives; Aggregates; Biocompatibility; Biotechnology; Burkholderia cepacia; Chemistry; Chemistry and Materials Science; Crosslinking; Design; Environmental Engineering/Biotechnology; Enzymes; Food Science; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Infrared analysis; Infrared spectra; Lignocellulose; Lipase; Olive oil; Polyethyleneimine; Reagents; Research Paper; Residues; Cross-linked enzyme aggregates; lipase; Palm fiber; Hydrolysis reactions; Burkholderia cepacia lipase
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-020-02419-0

A new design of cross-linked enzyme aggregates (CLEAs) of Burkholderia cepacia lipase (BCL) based mainly on the use of lignocellulosic residue of palm fiber as an additive was proposed. Different parameters for the preparation of active CLEAs in the hydrolysis of olive oil, such as precipitation agents, crosslinking agent concentration, additives, and coating agents were investigated. The highest activity yield (121.1 ± 0.1%) and volumetric activity (1578.1 ± 2.5 U/mL) were achieved for CLEAs prepared using the combination of a coating step with Triton ® X-100 and polyethyleneimine plus the use of palm fiber as an additive. The variations of the secondary structures of BCL-CLEAs were analyzed by second-derivative infrared spectra, mainly indicating a reduction of the α-helix structure, which was responsible for the lipase activation in the supramolecular structure of the CLEAs. Thus, these results provided evidence of an innovative design of BCL-CLEAs as a sustainable and biocompatible opportunity for biotechnology applications.