Antibacterial Potential of Biosynthesized Silver Nanoparticles Using Berberine Extract Against Multidrug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa

• Published in: Indian journal of microbiology Vol. 64; no. 1; pp. 125 - 132
• Main Authors: Tahan, Maedeh, Zeraatkar, Shadi, Neshani, Alireza, Marouzi, Parviz, Behmadi, Mostafa, Alavi, Seyed Jamal, Hashemi Shahri, Seyed Hamed, Hosseini Bafghi, Mahdi
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.03.2024; Springer Nature B.V
• Subjects: Acinetobacter baumannii; Antibacterial activity; Antibiotics; Antiinfectives and antibacterials; Bacteria; Bacterial diseases; Berberine; Biomedical and Life Sciences; Biosynthesis; Drug resistance; Life Sciences; Low concentrations; Medical Microbiology; Microbiology; Microorganisms; Minimum inhibitory concentration; Multidrug resistance; Nanoparticles; Original Research Article; Plant bacterial diseases; Plant extracts; Pseudomonas aeruginosa; Silver; Strains (organisms); Synergistic effect; Zeta potential; Multi-drug resistant; Biosynthesis; MIC; Silver nanoparticles; Pseudomonas aeruginosa; Acinetobacter baumannii
• ISSN: 0046-8991; 0973-7715
• DOI: 10.1007/s12088-023-01136-y

The emergence of multidrug resistance in bacterial infections has limited the use of antibiotics. Helping the action of antibiotics is one of the needs of the day. Today, the biosynthesis of nanoparticles (NPs) is considered due to its safety and cost-effectiveness. In this study, we investigated the effect of biosynthesized silver nanoparticles (AgNPs) by Berberine plant extract against standard strains of multidrug-resistant (MDR) Acinetobacter baumannii and Pseudomonas aeruginosa . Utilized UV–Vis, FTIR, FESEM/EDX, XRD, DLS, and Zeta potential techniques to confirm the biosynthesis of NPs. Then, disk diffusion agar (DDA) and minimum inhibitory concentration (MIC) tests were performed using common classes of standard antibiotics and AgNPs on the mentioned bacteria. The synergistic action between AgNPs and antibiotics was evaluated by the checkerboard method. First, we obtained the confirmation results of the biosynthesis of AgNPs. According to the DDA test, both standard bacterial strains were sensitive to NPs and had an inhibition zone. Also, the MIC values showed that AgNPs inhibit the growth of bacteria at lower concentrations than antibiotics. On the other hand, the results obtained from checkerboard monitoring showed that AgNPs, in combination with conventional antibiotics, have a synergistic effect. The advantage of this study was comparing the antibacterial effect of AgNPs alone and mixed with antibiotics. The antibacterial sensitivity tests indicated that the desired bacterial strains could not grow even in low concentrations of AgNPs. This property can be applied in future programs to solve the drug resistance of microorganisms in bacterial diseases.