Flavonoid-enrich extract of Autranella congolensis (Sapotaceae) protects against aluminium chloride-mediated Alzheimer’s disease-like oxidative stress in rat through the antioxidant properties

• Published in: Metabolic brain disease Vol. 38; no. 3; pp. 1025 - 1034
• Main Authors: Ngoumen, Dany Joël Ngassa, Mandob, Damaris Enyegue, Ella, Fils Armand, Ambamba, Bruno Dupon Akamba, Nanhah, Jules Vidal Kamga, Fonkoua, Martin, Ngondi, Judith Laure
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2023; Springer Nature B.V
• Subjects: 1-Butanol - pharmacology; Aluminum; Aluminum Chloride; Alzheimer Disease - chemically induced; Alzheimer Disease - drug therapy; Alzheimer Disease - prevention & control; Alzheimer's disease; Animals; Antioxidants; Antioxidants - therapeutic use; Biochemistry; Biomarkers; Biomedical and Life Sciences; Biomedicine; Brain damage; Brain injury; Butanol; Catalase; Damage prevention; Flavonoids; Flavonoids - pharmacology; Flavonoids - therapeutic use; Glutathione; Glutathione peroxidase; Lipid Peroxidation; Lipids; Metabolic Diseases; Neurodegenerative diseases; Neurology; Neurosciences; Oncology; Original Article; Oxidation; Oxidative Stress; Peroxidase; Peroxidation; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Proteins; Rats; Rats, Wistar; Sapotaceae - metabolism; Tocopherol; Vitamin E; Vitamin E - pharmacology; Redox state; Aluminium chloride; Flavonoids; Alzheimer disease; In vivo; Autranella congolensis
• ISSN: 0885-7490; 1573-7365
• DOI: 10.1007/s11011-022-01142-x

Oxidative stress (OS) is well established as a major event in Alzheimer’s disease (AD) pathology. One of the mostly-researched classes of antioxidants to manage with overwhelming OS include flavonoids. This study was aimed to investigate the protective effect of A. congolensis extract (HEEAC) on AlCl 3 -mediated AD-like OS and assess the contribution of its antioxidant flavonoid contents. Female Wistar (250–300 g) rats received orally 50 mg/Kg bw of AlCl 3 , followed one hour later by doses (150 or 300 mg/kg) of HEEAC or vitamin E at 100 mg/kg daily for eight consecutive weeks. OS related biomarkers were evaluated at the end of treatment. To assess the contribution of flavonoid contents to its activity, HEEAC was fractioned using solvent of varying polarities. Flavonoid-rich extracts obtained were tested for their antioxidant capacity. AlCl 3 administration significantly lowered antioxidant enzymes (catalase, glutathione peroxidase) and aconitase levels, reduced total thiol and thiol protein levels and increased lipid peroxidation and protein oxidation levels in brain. When co-administrated with HEEAC at 150 mg/kg, all of these OS related biomarkers were significantly moderated. The efficacity of the extract was significantly higher than vitamin E. Flavonoid-rich fractions extracted mainly n-butanol fraction show strong antioxidant activity, which can be considered as the major antioxidant fraction of this plant. HEEAC protect brain cells against oxidative damage induced by AlCl 3 , specifically through the strong antioxidant property of its n-butanol flavonoid-rich fraction, which may be a promising agent for preventing oxidative damage in AD.