Whole-Genome Methylation Sequencing of Large Yellow Croaker (Larimichthys crocea) Liver Under Hypoxia and Acidification Stress

• Published in: Marine biotechnology (New York, N.Y.) Vol. 25; no. 4; pp. 567 - 579
• Main Authors: Yue, Yanfeng, Wang, Yabing, Zhang, Bianbian, Zeng, Jiao, Wang, Qian, Wang, Cuihua, Peng, Shiming
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.08.2023; Springer Nature B.V
• Subjects: Acidification; Adhesion; Aldosterone; Analysis; Aquaculture; Biological stress; Biomedical and Life Sciences; Cell adhesion; Cell adhesion & migration; Cell adhesion molecules; Chemical synthesis; DNA methylation; Encyclopedias; Engineering; Enrichment; Fish; Freshwater & Marine Ecology; Gene sequencing; Genes; Genomes; Hormones; Hypoxia; Immune system; Immunity; Larimichthys crocea; Life Sciences; Liver; Microbiology; Nervous system; Physiological responses; Polyculture (aquaculture); Receptors; Secretion; Sequencing; Signal transduction; Stress response; Systems development; Tissue; Whole genome sequencing; Zoology; Acidification; Hypoxia; Methylation; Liver; Large yellow croaker; Larimichthys crocea
• ISSN: 1436-2228; 1436-2236
• DOI: 10.1007/s10126-023-10226-3

Large yellow croaker ( Larimichthys crocea ) is an important aquaculture species in China. This study analysed whole-genome methylation differences in liver tissues of young fish under different hypoxic and acidification conditions. Differentially methylated regions (DMRs) and differentially methylated genes (DMGs) were identified. Gene ontology (GO) and Kyoto encyclopaedia of genes and genomes (KEGG) enrichment analyses of DMGs were conducted to explore the mechanism of coping with hypoxic acidification. The main methylation type was CG, accounting for > 70% of total methylation, significantly higher than CHG and CHH methylation types. GO enrichment analysis of DMGs revealed strong enrichment of nervous system development, cell periphery, plasma membrane, cell junction organisation, cell junction, signalling receptor activity, molecular sensor activity, cell-linked tissue junction organisation, cell–cell adhesion and nervous system development. KEGG enrichment analysis of DMR-related genes identified cell adhesion molecules, cortisol synthesis and secretion and aldosterone synthesis and secretion as the three key pathways regulating the physiological responses to hypoxia and acidification. Long-term hypoxic and acidification stress affected the immune system, nervous system and stress responses of large yellow croaker. Whole-genome sequencing analysis of exposed tissues was used to investigate changes that occur in L. crocea i n response to hypoxic and acidic conditions at the DNA methylation level. The findings contribute to our comprehensive understanding of functional methylation in large yellow croaker and will support future research on the response mechanisms of this species under different environmental pressures.