Electronic Quantification of Protein Biomarkers Based on Bead Aggregate Sizing

We demonstrate a novel protein sensing method using detection of bead aggregates of different sizes in a micro-fluidic impedance cytometer. We utilize micro-fabricated coplanar electrodes at the base of our micro-channel to measure the impedance. When the single beads or bead aggregates pass through...

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Bibliographic Details
Published inIEEE sensors journal Vol. 15; no. 12; pp. 6763 - 6764
Main Authors Zhongtian Lin, Pengfei Xie, Xinnan Cao, Javanmard, Mehdi
Format Journal Article
LanguageEnglish
Published New York IEEE 01.12.2015
The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subjects
Aggregates
Amplifiers
Beads
Biomarkers
biosensing
cytometry
Electrodes
Electronics
Impedance
Microfluidics
protein detection
Proteins
Sensors
protein detection
cytometry
microfluidics
Biosensing
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Summary:We demonstrate a novel protein sensing method using detection of bead aggregates of different sizes in a micro-fluidic impedance cytometer. We utilize micro-fabricated coplanar electrodes at the base of our micro-channel to measure the impedance. When the single beads or bead aggregates pass through the electrodes, a difference in impedance change is detected. We measure these signals using a lock-in amplifier. Due to the difference in impedance change for unbound beads compared with protein-bound bead aggregates, we can differentiate the two types from each other electronically. We demonstrate proof-of-concept using streptavidin and biotin coated beads.
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ISSN:1530-437X
1558-1748
DOI:10.1109/JSEN.2015.2458292