A novel immunoassay of smooth muscle myosin heavy chain in serum

We have developed a double monoclonal sandwich enzyme immunoassay to measure smooth muscle myosin heavy chain (MHC). Analytical performance of the assay showed reliable detection of smooth muscle MHC in human sera. The mean of the smooth muscle MHC level in normal human sera was 0.9 ± 0.9 ng/ml. In...

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Bibliographic Details
Published inJournal of immunological methods Vol. 185; no. 1; pp. 57 - 63
Main Authors Katoh, Hirohisa, Suzuki, Toru, Yokomori, Kinji, Suzuki, Shin, Ohtaki, Eiji, Watanabe, Masafumi, Yazaki, Yoshio, Nagai, Ryozo
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 11.09.1995
Subjects
Aneurysm, Dissecting - blood
Aneurysm, Dissecting - diagnosis
Animals
Antibodies, Monoclonal - immunology
Antibody Specificity
Aortic dissection
Cross Reactions
ELISA
Humans
Immunoassay
Mice
Mice, Inbred BALB C
Molecular Weight
Monoclonal antibody
Muscle, Smooth
Myosins - blood
sandwich
Smooth muscle myosin heavy chain
PBS
BSA
TMB
Aortic dissection
MHC
Monoclonal antibody
Smooth muscle myosin heavy chain
ELISA
MAb
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Summary:We have developed a double monoclonal sandwich enzyme immunoassay to measure smooth muscle myosin heavy chain (MHC). Analytical performance of the assay showed reliable detection of smooth muscle MHC in human sera. The mean of the smooth muscle MHC level in normal human sera was 0.9 ± 0.9 ng/ml. In sera of patients with aortic dissection, the smooth muscle MHC level sharply elevated at the onset and rapidly decreased to normal levels. Immunoassay of smooth muscle MHC in serum is a promising method for biochemical diagnosis of smooth muscle disorders.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(95)00104-I