Overexpression of RPOTmp Being Targeted to Either Mitochondria or Chloroplasts in Arabidopsis Leads to Overall Transcriptome Changes and Faster Growth

The transcription of Arabidopsis organellar genes is performed by three nuclear-encoded RNA polymerases: RPOTm, RPOTmp, and RPOTp. The RPOTmp protein possesses ambiguous transit peptides, allowing participation in gene expression control in both mitochondria and chloroplasts, although its function i...

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Published inInternational journal of molecular sciences Vol. 25; no. 15; p. 8164
Main Authors Gorbenko, Igor V., Tarasenko, Vladislav I., Garnik, Elena Y., Yakovleva, Tatiana V., Katyshev, Alexander I., Belkov, Vadim I., Orlov, Yuriy L., Konstantinov, Yuri M., Koulintchenko, Milana V.
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.08.2024
Subjects
anterograde/retrograde regulation
Arabidopsis thaliana
Chloroplasts
Gene expression
Genes
Genetic engineering
Genomes
Instrument industry
International economic relations
Metabolism
Metabolites
Mitochondria
Mitochondrial DNA
NAC transcription factors
nuclear-encoded RNA polymerases (NEPs)
organellar gene expression (OGE)
Plant growth
plant transcriptome
Plastids
Proteins
RNA polymerase
Seeds
Transcription factors
Transfer RNA
United States
Russia
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Summary:The transcription of Arabidopsis organellar genes is performed by three nuclear-encoded RNA polymerases: RPOTm, RPOTmp, and RPOTp. The RPOTmp protein possesses ambiguous transit peptides, allowing participation in gene expression control in both mitochondria and chloroplasts, although its function in plastids is still under discussion. Here, we show that the overexpression of RPOTmp in Arabidopsis, targeted either to mitochondria or chloroplasts, disturbs the dormant seed state, and it causes the following effects: earlier germination, decreased ABA sensitivity, faster seedling growth, and earlier flowering. The germination of RPOTmp overexpressors is less sensitive to NaCl, while rpotmp knockout is highly vulnerable to salt stress. We found that mitochondrial dysfunction in the rpotmp mutant induces an unknown retrograde response pathway that bypasses AOX and ANAC017. Here, we show that RPOTmp transcribes the accD, clpP, and rpoB genes in plastids and up to 22 genes in mitochondria.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25158164