Effects of resveratrol and its analogue pterostilbene, on NOV/CCN3 adipokine in adipose tissue from rats fed a high-fat high-sucrose diet

• Published in: Journal of physiology and biochemistry Vol. 75; no. 3; pp. 275 - 283
• Main Authors: Trepiana, J., Gómez-Zorita, S., Fernández-Quintela, Alfredo, González, M., Portillo, M. P.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.08.2019; Springer Nature B.V
• Subjects: Adipose tissue; Adipose Tissue - drug effects; Adipose Tissue - metabolism; Adiposity - drug effects; Animal Physiology; Animals; Biomedical and Life Sciences; Biomedicine; Diet; Diet, Carbohydrate Loading; Diet, High-Fat; Dietary supplements; Gene expression; High fat diet; Human Physiology; Immediate-Early Proteins - metabolism; Male; Obesity; Obesity - drug therapy; Obesity - metabolism; Original Article; PGC-1 protein; Phenolic compounds; Phenols; Protein expression; Proteins; Rats; Rats, Wistar; Resveratrol; Resveratrol - pharmacology; Rodents; Stilbenes - pharmacology; Sucrose; Weight; NOV/CCN3; Obesity; Adipose tissue; Phenolic compounds; Pterostilbene; Resveratrol
• ISSN: 1138-7548; 1877-8755
• DOI: 10.1007/s13105-019-00680-w

Nephroblastoma overexpressed protein, also called NOV/CCN3, is an adipokine which is present in various tissues and recently linked to obesity. The objective of the study was to determine the effect of resveratrol and pterostilbene on NOV/CCN3 in adipose tissue from rats fed an obesogenic diet. Thirty-six male Wistar rats were split into four groups ( n  = 9): fed a standard diet (CC), high-fat high-sucrose (HFS) diet supplemented with resveratrol (RSV; 30 mg/kg/day) or with pterostilbene (PT; 30 mg/kg/day), or without phenolic supplementation (HFS). Rats were sacrificed after 6 weeks of treatment, and adipose tissue (white and brown) from different anatomical locations were dissected. Then, Nov/ccn3 gene and protein expression and the adipogenic genes, Ucp-1 and Pgc-1a , expressions were studied. Increased weight of white adipose tissues was found in rats fed the HFS diet. Whereas resveratrol-treated rats showed reduced internal and total adipose tissue weights, pterostilbene-treated rats showed reduced subcutaneous, internal and total adipose depots. Nov/ccn3 gene expression decreased in epididymal and interscapular brown depot in rats fed HFS diet when compared with the control group. Regarding the phenolic compounds, resveratrol prompted a Nov/ccn3 gene expression increase in epididymal fat tissue, whereas pterostilbene reduced its protein expression compared with the obese group. However, these phenolic compounds did not affect NOV/CCN3 expression in brown depot. NOV/CCN3 seems to be involved in weight changes in epididymal adipose tissue under obesogenic feeding, but not in subcutaneous, acting as a protective mechanism counteracting the fattening effect of the diet. To our knowledge, this is the first study analyzing whether NOV/CCN3 is involved in the anti-obesity effect of resveratrol and pterostilbene. Our results suggest that this is not the case.