Changes in the Transcription of Proliferation- and Apoptosis-Related Genes in Embryos in Women of Different Ages under the Influence of Extracellular Vesicles from Donor Follicular Fluid In Vitro

• Published in: Bulletin of experimental biology and medicine Vol. 176; no. 5; pp. 658 - 665
• Main Authors: Nepsha, O. S., Burmenskaya, O. V., Akhmedova, Z. F., Romanov, E. A., Sysoeva, A. P., Goryunov, K. V., Shevtsova, Yu. A., Silachev, D. N., Makarova, N. P., Kalinina, E. A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2024; Springer Nature B.V
• Subjects: Age; Apoptosis; Aquaporin 3; BAX gene; Biomedical and Life Sciences; Biomedicine; Blastocysts; Cell Biology; Cell culture; Embryos; Extracellular vesicles; Follicular fluid; Gene expression; Genes; Gynecology; Internal Medicine; Laboratory Medicine; Medical research; Nanoparticles; Obstetrics; Particle size; Pathology; Proteins; Software; Vascular endothelial growth factor; embryo; mRNA; extracellular vesicles; co-culturing; follicular fluid
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-024-06087-y

We studied the influence of extracellular vesicles from the follicular fluid of a young donor on gene expression ( MKI67 , MYBL2 , CCNB1 , CCND1 , C CNE1 , CALM2 , BAX , NDRG1 , TP53I3 , VEGF , VCAN , HAS2 , CTSL2 , PIBF1 , RPL37 , PFKP , GPX3 , and AQP3 ) in embryos of women of different ages. According to nanoparticle tracking analysis data, the concentration of extracellular vesicles was 3.75±0.47×10 11 particles/ml and the mean particle size was 138.78±9.90 nm. During co-culturing of the follicular fluid extracellular vesicles with blastocysts of young women, we observed significantly increased expression of mRNA for genes CTSL2 , CCND1 , CCNE1 , VEGF and reduced expression of BAX gene mRNA in comparison with embryos in women of late reproductive age. We hypothesized that addition of extracellular vesicles of the oocyte follicular fluid from a young donor to the culture medium of embryos could slow down apoptosis process typical of blastocyst cells in women above 36 years.