Nuclear myosin is ubiquitously expressed and evolutionary conserved in vertebrates

• Published in: Histochemistry and cell biology Vol. 127; no. 2; pp. 139 - 148
• Main Authors: Kahle, M, Pridalová, J, Spacek, M, Dzijak, R, Hozák, P
• Format: Journal Article
• Language: English
• Published: Germany Springer Nature B.V 01.02.2007
• Subjects: Amino Acid Sequence; Animals; Cell Line; Cell Nucleus - metabolism; Cellular biology; Chromatin; Conserved Sequence; Female; Gene Expression; Humans; Male; Mice; Myosin Type I - chemistry; Myosin Type I - genetics; Myosin Type I - isolation & purification; Myosin Type I - metabolism; Nucleic Acid Amplification Techniques; Phylogeny; Sequence Homology, Nucleic Acid; Serum - chemistry; Transcription, Genetic; Vertebrates; Vertebrates - genetics; Vertebrates - metabolism
• ISSN: 0948-6143; 1432-119X
• DOI: 10.1007/s00418-006-0231-0

Nuclear myosin I (NMI) is a single-headed member of myosin superfamily localized in the cell nucleus which participates along with nuclear actin in transcription and chromatin remodeling. We demonstrate that NMI is present in cell nuclei of all mouse tissues examined except for cells in terminal stages of spermiogenesis. Quantitative PCR and western blots demonstrate that the expression of NMI in tissues varies with the highest levels in the lungs. The expression of NMI is lower in serum-starved cells and it increases after serum stimulation. The lifespan of NMI is longer than 16 h as determined by cycloheximide translation block. A homologous protein is expressed in human, chicken, Xenopus, and zebrafish as shown by RACE analysis. The analysis of genomic sequences indicates that almost identical homologous NMI genes are expressed in mammals, and similar NMI genes in vertebrates.