Increased glucose dependence in resting, iron-deficient rats

Rates of blood glucose and lactate turnover were assessed in resting iron-deficient and iron-sufficient (control) rats to test the hypothesis that dependence on glucose metabolism is increased in iron deficiency. Male Sprague-Dawley rats, 21 days old, were fed a diet containing either 6 mg iron/kg f...

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Published inThe American journal of physiology Vol. 253; no. 4; p. E461
Main Authors Brooks, G.A, Henderson, S.A, Dallman, P.R
Format Journal Article
LanguageEnglish
Published United States 01.10.1987
Subjects
Animals
Basal Metabolism
Blood Glucose - metabolism
CATECHOLAMINE
CATECHOLAMINES
CATECOLAMINAS
CONSOMMATION D'OXYGENE
CONSUMO DE OXIGENO
DEFICIENCY DISEASES
Energy Metabolism
ENFERMEDADES CARENCIALES
FER
GLUCOSA
GLUCOSE
HIERRO
IRON
Iron - deficiency
Kinetics
Lactates - blood
Lactic Acid
MALADIE DE CARENCE
Male
Mathematics
OXYGEN CONSUMPTION
RAT
RATA
RATS
Rats, Inbred Strains
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Summary:Rates of blood glucose and lactate turnover were assessed in resting iron-deficient and iron-sufficient (control) rats to test the hypothesis that dependence on glucose metabolism is increased in iron deficiency. Male Sprague-Dawley rats, 21 days old, were fed a diet containing either 6 mg iron/kg feed (iron-deficient group) or 50 mg iron/kg feed (iron-sufficient group) for 3-4 wk. The iron-deficient group became anemic, with hemoglobin levels of 6.4 +/- 0.2 compared with 13.8 +/- 0.3 g/dl for controls. Rats received a 90-min primed continuous infusion of D-[6-3H]glucose and sodium L-[U-14C]lactate via a jugular catheter. Serial samples were taken from a carotid catheter for concentration and specific activity determinations. Iron-deficient rats had significantly (P less than 0.05) higher blood glucose (7.1 +/- 0.3 vs. 6.1 +/- 0.2 mM) and lactate concentrations than controls (1.0 +/- 0.1 vs. 0.8 +/- 0.1 mM). The iron-deficient group had a significantly higher glucose turnover rate (67 +/- 2 vs. 58 +/- 4 mumol . kg-1 . min-1) than the control group. Significantly more metabolite recycling in iron-deficient rats was indicated by greater incorporation of 14C (from infused [14C]-lactate) into blood glucose. Assuming a carbon crossover correction factor of 2, half of blood glucose arose from lactate in deficient animals. By comparison, only 25% of glucose arose from lactate in controls. Lack of a difference in lactate turnover (irreversible disposal) rates between deficient rats and controls (191 +/- 26 vs. 163 +/- 15 mumol . kg-1 . min-1) was attributed to 14C recycling.
Bibliography:S30
881033388
ISSN:0002-9513
2163-5773
DOI:10.1152/ajpendo.1987.253.4.E461