Credible Serological Evidence of Latent Toxoplasma Infection Among Women with Primary Infertility: A Ten-Year Registry-Based Study

• Published in: Acta parasitologica Vol. 68; no. 2; pp. 400 - 405
• Main Authors: Zamaniyan, Marzieh, Fakhar, Mahdi, Tabaripour, Rabeeh, Peivandi, Saloumeh, Keighobadi, Masoud, Ghasemi, Samira, Montazeri, Mahbobeh
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.06.2023; Springer Nature B.V
• Subjects: Abortion; Animal Systematics/Taxonomy/Biogeography; Antibodies; Antibodies, Protozoan; Baseline studies; Biomedical and Life Sciences; Biomedicine; Chronic infection; Contraceptives; Ecology; Enzyme-linked immunosorbent assay; Female; Hormones; Humans; Immunoglobulin G; Immunoglobulin M; In vitro fertilization; Infections; Infertility; Infertility, Female - epidemiology; Iran - epidemiology; Medical Microbiology; Medical science; Microbiology; Original Paper; Parasitology; Polycystic ovary syndrome; Population studies; Prolactin; Retrospective Studies; Seroepidemiologic Studies; Serology; Serum levels; Sorbents; Statistical analysis; Toxoplasma; Toxoplasmosis; Toxoplasmosis - complications; Toxoplasmosis - epidemiology; Iran; Iran; Latent toxoplasmosis; Primary infertility; Toxoplasma gondii
• ISSN: 1230-2821; 1896-1851; 1896-1851
• DOI: 10.1007/s11686-023-00677-y

Background Some evidence reveled that chronic infection with Toxoplasma gondii ( T. gondii ) has recently been associated with infertility in human and experimental model . This baseline study aimed to investigate serological evidence of Toxoplasma infection among infertile women who admitted to the in vitro fertilization (IVF) clinic at Imam Khomeini Hospital, Mazandaran province, Sari, northern Iran. Subjects and Methods In this retrospective (descriptive-analytical) study, all infertile women referred to the IVF clinic during 2010–2019 (10 years), constitute the study population. All data including demographic and some related characteristics were collected into a questionnaire and registered at the Iranian National Registry Center for Toxoplasmosis (INRCT) at the Mazandaran University of Medical Sciences, northern Iran. The existence of anti- Toxoplasma antibodies (IgG and IgM) was explored using a commercially available enzyme-linked immune sorbent assay (ELISA) kit (PishtazTeb, Iran), based on the manufacturer’s protocol. Results Of 520 infertile women, anti- T. gondii IgG, IgM and both IgG and IgM antibodies were detected among 342/520 (65.77%), 1/520 (0.19) and 4/520 (0.77) infertile women, respectively. Primary and secondary infertility was detected in 74.56% and 25.44% of IgG seropositive infertile women, respectively. Also, most of the IgG seropositive subjects had no history of abortion, polycystic ovary syndrome (PCO), fibroma, contraceptive use and varicocele in spouse as primary cause of infertility. Furthermore, serum levels of prolactin and antimullerian (AMH) hormones were normal in 81.29 and 80.12% of infertile women with anti- T. gondii IgG, respectively. There was also a statistically significant difference between the seroprevalence of Toxoplasma infection and these variables associated to primary infertility ( P  < 0.05). Conclusion According to the high prevalence (about two thirds) of chronic T. gondii infection among infertile women, particularly those with a history of abortion and primary infertility, it can be concluded that latent Toxoplasma infection pose a risk to infertile woman in the study area. Therefore, we advise that screening and treatment of Toxoplasma infection among infertile women must be favorably considered.