Flavobacterium psychrotrophum sp. nov. and Flavobacterium panacagri sp. nov., Isolated from Freshwater and Soil

• Published in: The journal of microbiology Vol. 61; no. 10; pp. 891 - 901
• Main Authors: Kim, Yong-Seok, Hwang, Eun-Mi, Jeong, Chang-Myeong, Cha, Chang-Jun
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01.10.2023; Springer Nature B.V
• Subjects: Bacterial Typing Techniques; Biomedical and Life Sciences; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - analysis; Flavobacterium; Fresh Water - microbiology; Freshwater; Gene sequencing; Genomic analysis; Gliding; Hybridization; Inland water environment; Life Sciences; Lipids; Menaquinones; Microbial Systematics and Evolutionary Microbiology; Microbiological strains; Microbiology; New species; Nucleotide sequence; Nucleotides; Phosphatidylethanolamine; Phylogenetics; Phylogeny; Pigments; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sodium chloride; Soil; Soil microorganisms; Soils; Strains (organisms); Vitamin K 2; Novel species; Genome; Flavobacterium panacagri; Flavobacterium; Flavobacterium psychrotrophum
• ISSN: 1225-8873; 1976-3794; 1976-3794
• DOI: 10.1007/s12275-023-00081-1

Two novel bacterial strains CJ74 T and CJ75 T belonging to the genus Flavobacterium were isolated from freshwater of Han River and ginseng soil, South Korea, respectively. Strain CJ74 T was Gram-stain-negative, aerobic, rod-shaped, non-motile, and non-flagellated, and did not produce flexirubin-type pigments. Strain CJ75 T was Gram-stain-negative, aerobic, rod-shaped, motile by gliding, and non-flagellated, and produced flexirubin-type pigments. Both strains were shown to grow optimally at 30 °C in the absence of NaCl on R2A medium. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains CJ74 T and CJ75 T belonged to the genus Flavobacterium and were most closely related to Flavobacterium niveum TAPW14 T and Flavobacterium foetidum CJ42 T with 96.17% and 97.29% 16S rRNA sequence similarities, respectively. Genomic analyses including the reconstruction of phylogenomic tree, average nucleotide identity, and digital DNA-DNA hybridization suggested that they were novel species of the genus Flavobacterium . Both strains contained menaquinone 6 (MK-6) as the primary respiratory quinone and phosphatidylethanolamine as a major polar lipid. The predominant fatty acids of both strains were iso-C 15:0 and summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c ). Based on the polyphasic taxonomic study, strains CJ74 T and CJ75 T represent novel species of the genus Flavobacterium , for which names Flavobacterium psychrotrophum sp. nov. and Flavobacterium panacagri sp. nov. are proposed, respectively. The type strains are CJ74 T (=KACC 19819 T  =JCM 32889 T ) and CJ75 T (=KACC 23149 T  =JCM 36132 T ).