Overexpression of Synoviolin and miR-125a-5p, miR-19b-3p in peripheral blood of rheumatoid arthritis patients after treatment with conventional DMARDs and methylprednisolone
Purpose SYVN1 is an endoplasmic reticulum (ER)-resident E3 ubiquitin ligase that has an essential function along with SEL1L in rheumatoid arthritis (RA) pathogenesis. This study aimed to investigate the changes in the expression of peripheral blood ncRNAs and SYVN1-SEL1L affected by DMARDs treatment...
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Published in | Clinical rheumatology Vol. 43; no. 1; pp. 147 - 157 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Cham
Springer International Publishing
2024
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Purpose
SYVN1
is an endoplasmic reticulum (ER)-resident E3 ubiquitin ligase that has an essential function along with
SEL1L
in rheumatoid arthritis (RA) pathogenesis. This study aimed to investigate the changes in the expression of peripheral blood ncRNAs and
SYVN1-SEL1L
affected by DMARDs treatment.
Methods
Twenty-five newly diagnosed RA patients were randomly assigned to receive conventional DMARDs (csDMARDs) and methylprednisolone for six months. The peripheral blood gene expression of
SYVN1
and
SEL1L
and possible regulatory axes
, NEAT1, miR-125a-5p,
and
miR-19b-3p,
were evaluated before and after qRT-PCR. We also compared differences between the patients and healthy controls (HCs), and statistical analyses were performed to determine the correlation between ncRNAs with
SYVN1-SEL1L
and the clinical parameters of RA.
Results
Expression of
NEAT1
(
P
= 0.0001)
,
miR
-19b-3p
(P = 0.007),
miR-125a-5p
(
P
= 0.005), and
SYVN1
(
P
= 0.036) was significantly increased in newly diagnosed patients compared to HCs; also,
miR-125a-5p, miR-19b-3p,
and
SYVN1 were
significantly overexpressed after treatment (
P
= 0.001,
P
= 0.001, and
P
= 0.005, respectively).
NEAT1
was positively correlated with
SYVN1,
and
miR-125a-5p
had a negative correlation with anti-cyclic citrullinated peptides. The ROC curve analysis showed the potential role of selected ncRNAs in RA pathogenesis.
Conclusion
The results indicate the ineffectiveness of the csDMARDs in reducing SYVN1 expression. The difference in expression of ncRNAs might be useful markers for monitoring disease activity and determining therapeutic responses in RA patients.
Key Points
• The expression of NEAT1 is significantly upregulated in RA patients compared to HC subjects.
• miR-19b-3p, miR-125a-5p, and SYVN1 are significantly upregulated in RA patients compared to HC subjects.
• The expression of miR-19b-3p and miR-125a-5p is significantly increased in RA patients after treatment with DMARDs and methylprednisolone.
• NEAT1 is positively correlated with SYVN1. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Undefined-3 |
ISSN: | 0770-3198 1434-9949 |
DOI: | 10.1007/s10067-023-06808-0 |