Bacterial cellulose production by Komagataeibacter hansenii can be improved by successive batch culture

• Published in: Brazilian journal of microbiology Vol. 54; no. 2; pp. 703 - 713
• Main Authors: Gomes, Rodrigo José, Ida, Elza Iouko, Spinosa, Wilma Aparecida
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.06.2023; Springer Nature B.V
• Subjects: Acetobacteraceae - genetics; Bacteria; Batch Cell Culture Techniques; Batch culture; Biomedical and Life Sciences; Biopolymers; Biosynthesis; Biotechnology and Industrial Microbiology - Research Paper; Cell culture; Cellulose; Fermentation; Food Microbiology; Life Sciences; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Mycology; Thermal properties; Thermodynamic properties; Work capacity; Method development; Acetic fermentation; Acetic acid bacteria; Biopolymer
• ISSN: 1517-8382; 1678-4405
• DOI: 10.1007/s42770-023-00910-w

Bacterial cellulose (BC) is a biopolymer principally synthetized by strains of the genus Komagataeibacter . However, high costs and low production yield make large-scale application difficult. The aim of this work was to evaluate the effects of successive batch culture before fermentation on the ability to increase the capacity of bacterial cellulose biosynthesis by a low-producing strain. The Komagataeibacter hansenii strain ATCC 23,769 was initially cultivated in fermentation broth for two periods of 35 or 56 days under static conditions. At the end of each period of time, they were transferred to new broth to be cultivated again (new batch culture cycle) for 35 or 56 days and carried out in parallel with a 10-day fermentation to determine the quantity of BC produced. As a result, a greater increase was observed after the end of the second and third batch cultures of 56 days (increases of 137% and 187% in relation to the nonbatch cultured strain, respectively). The produced samples presented higher crystallinity and thermal properties but lower water holding capacity. Through this work, it was concluded that the longer the batch culture time was, the greater the increase in the capacity of cellulose biosynthesis, which also depended on the number of successive batch culture cycles carried out.