Gold nanoparticles (AuNPs) can rapidly deliver artificial microRNA (AmiRNA)-ATG6 to silence ATG6 expression in Arabidopsis
Key message We establish a fast and efficient transient silencing system that facilitates functional studies of some genes, whose knockout leads to plant lethality. In plants, the generation of loss-of-function mutants is crucial for studying gene function. Artificial microRNA (AmiRNA) technology is...
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Published in | Plant cell reports Vol. 42; no. 7; pp. 1191 - 1201 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.07.2023
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Key message
We establish a fast and efficient transient silencing system that facilitates functional studies of some genes, whose knockout leads to plant lethality.
In plants, the generation of loss-of-function mutants is crucial for studying gene function. Artificial microRNA (AmiRNA) technology is a more targeted and effective tool for gene silencing. Gold nanoparticles (AuNPs) can bind nucleic acids and deliver them into animal cells. Here, AuNPs are used in combination with AmiRNA technology in plants. We found that AmiRNA–
autophagy
-
related proteins
(
ATG6
) can be delivered to cells by AuNPs to achieve the effect of
ATG6
silencing. It is worth noting that on the 10th day there is still a silencing effect. Similar to the
atg5
lines, silencing of
ATG6
significantly reduced plant resistance to
Pseudomonas syringae pv.maculicola
(
Psm
) ES4326/
AvrRpt2.
Interestingly,
ATG6
silencing and
ATG5
mutation in
NPR1–GFP
(
nonexpressor of pathogenesis-related genes
) lines significantly reduced plant resistance to
Psm
ES4326/
AvrRpt2
, suggesting that autophagy is also involved in NPR1-regulated plant immune responses. In summary, we establish a fast and efficient transient silencing system that facilitates functional studies of some genes, whose knockout leads to plant lethality. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0721-7714 1432-203X |
DOI: | 10.1007/s00299-023-03026-5 |