Complete genome analysis of a novel narnavirus in sweet viburnum (Viburnum odoratissimum)

• Published in: Archives of virology Vol. 169; no. 5; p. 90
• Main Authors: Gao, Yangwei, Xu, Zhongtian, Li, Ping, Hu, Xiaodi, Chen, Jian-Ping, Zhang, Chuan-Xi, Li, Yiyuan
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.05.2024; Springer Nature B.V
• Subjects: Amino acid sequence; Amino acids; Annotated Sequence Record; Bats; Biomedical and Life Sciences; Biomedicine; Chloroplasts; DNA-directed RNA polymerase; Flowers & plants; Genome, Viral; Genomes; Infectious Diseases; Medical Microbiology; Nucleotide sequence; Open Reading Frames; Phylogenetics; Phylogeny; Proteins; RNA polymerase; RNA viruses; RNA Viruses - genetics; RNA, Viral - genetics; RNA-directed RNA polymerase; RNA-mediated interference; Shrubs; siRNA; Trees; Viburnum - genetics; Viburnum odoratissimum; Virology; Viruses
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-024-06000-y

Trees and shrubs provide important ecological services. However, few studies have surveyed the virome in trees and shrubs. In this study, we discovered a new positive-sense RNA virus originating from Viburnum odoratissimum , which we named "Vo narna-like virus". The complete genome of Vo narna-like virus is 3,451 nt in length with an open reading frame (ORF) encoding the RNA-dependent RNA polymerase (RdRP) protein. Phylogenetic analysis placed this virus within the betanarnavirus clade, sharing 53.63% amino acid sequence identity with its closest relative, Qingdao RNA virus 2. The complete sequence of the virus was confirmed by rapid amplification of cDNA ends (RACE) and Sanger sequencing. Small interfering RNA (siRNA) analysis indicated that this virus interacts with the RNA interference (RNAi) pathway of V. odoratissimum . This is the first report of a narnavirus in V. odoratissimum .