Neuroprotective effects of methyl jasmonate in male Wistar rats exposed to delayed acetic acid-induced ulcerative colitis: involvement of antioxidant status, GFAP, and IBA-1 immunoreactivities

• Published in: Metabolic brain disease Vol. 38; no. 2; pp. 671 - 686
• Main Authors: Omayone, Tosan P., Salami, Faizah T., Aluko, Oritoke M., Nathanniel, Jannie N., Jeje, Sikirullai O., Adedeji, Temitope G., Ijomone, Omamuyovwi M.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.02.2023; Springer Nature B.V
• Subjects: Acetic acid; Acetic Acid - toxicity; Amygdala; Animal models; Animals; Antioxidants; Antioxidants - pharmacology; Antioxidants - therapeutic use; Anxiety; Biochemistry; Biomedical and Life Sciences; Biomedicine; Colitis - chemically induced; Colitis - pathology; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - drug therapy; Colitis, Ulcerative - pathology; Colon - pathology; Glial fibrillary acidic protein; Hippocampus; Histology; Immunoreactivity; Indomethacin; Inflammatory bowel disease; Inflammatory bowel diseases; Male; Males; Mental depression; Metabolic Diseases; Methyl jasmonate; Neurology; Neuroprotection; Neuroprotective Agents - pharmacology; Neuroprotective Agents - therapeutic use; Neurosciences; Oncology; Original Article; Oxidants; Oxidizing agents; Rats; Rats, Wistar; Regeneration; Ulcerative colitis; Immunohistochemistry; Depression; Anxiety; Memory; Methyl jasmonate; Ulcerative colitis
• ISSN: 0885-7490; 1573-7365
• DOI: 10.1007/s11011-022-01145-8

Neurobehavioral deficits have been severally reported as a comorbid outcome in inflammatory bowel diseases (IBDs). This study evaluated neurological changes in the experimental model of IBDs, as well potential protective effects of methyl jasmonate (MJ). The study used the acetic acid model of colitis and thereafter delayed the healing process by the administration of indomethacin (Indo) (2 mg/kg, SC). Thirty male Wistar rats (120-160 g) were divided into 5 groups ( n  = 6). Control, Colitis, Colitis + Indo, MJ (50 mg/kg, IP) + Colitis and MJ + Colitis + Indo. Colitis was induced by intrarectal administration of 2 mL, 4% acetic acid. Neurobehavioral studies were carried out to assess memory function, depression, and anxiety on day 7 of post-colitis induction. Animals were thereafter sacrificed to collect the brain tissues for routine histology, immunoreactivity of GFAP and IBA-1, and biochemical assays. Neurobehavioral tests showed anxiety, depression, and memory deficits, especially in the Colitis + Indo group which were accompanied by increased IBA-1 and GFAP count. MJ reversed these effects and reduced GFAP count in the hippocampus and amygdala as well as IBA-1 count in the hippocampus, amygdala, and cortex. Histological observations of these areas showed no significant histopathological changes across all groups. GPx and CAT levels were significantly reduced, while MPO was significantly increased in colitis and Colitis+indo groups when compared with control, which was attenuated in groups administered with MJ. These findings tuggest that MJ possesses neuroprotective, anti-oxidant, and neuron-regeneration properties. Therefore, it could be considered as a potential treatment for behavioral deficits associated with ulcerative colitis.