Anti-insomnia Effect of a Polyherbal Formulation on P-chlorophenyalanine Induced Experimental Animal Model

• Published in: Neurochemical research Vol. 49; no. 2; pp. 327 - 337
• Main Authors: Sheethal, S, Ratheesh, M, Jose, Svenia P, Sandya, S, Samuel, Sarala, Madhavan, Jayashree
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.02.2024; Springer Nature B.V
• Subjects: Animal behavior; Animal models; Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell Biology; Hormones; Inflammation; Insomnia; Lipid peroxidation; Lipids; Models, Animal; Neurochemistry; Neurology; Neurosciences; Neurotransmitter Agents; Neurotransmitters; Neurotrophic factors; Original Paper; Oxidative stress; Peroxidation; Rats; Receptors; Side effects; Sleep - physiology; Sleep disorders; Sleep Initiation and Maintenance Disorders - chemically induced; Sleep Initiation and Maintenance Disorders - drug therapy; Oxidative stress; Neurotransmitters; Inflammation; Insomnia; neurotrophins
• ISSN: 0364-3190; 1573-6903
• DOI: 10.1007/s11064-023-04035-2

Sleep is a dynamic and controlled set of physiological and behavioural practices during which the stabilisation and restoration processes of the body take place properly. Therefore, sleep disorders, especially chronic insomnia, can harm an individual’s physical and mental health. However, the therapeutic alternatives are limited and possess severe side effects. Thus, in this study, we aimed to investigate the anti-insomnia effect of a polyherbal formulation (Sleep) (SLP) on p-chlorophenyalanine (PCPA) induced insomnia in rats. Intraperitoneal injection of PCPA induced the experimental condition, and the therapeutic effect of SLP was evaluated by studying the sleep pattern and expression of various neurotransmitters and receptors, along with neurotrophins. Moreover, insomnia-associated oxidative stress and inflammation were also studied. From the findings, we found that the SLP-supplemented animals improved their sleeping behaviour and that the major neurotransmitters, hormones, and receptors were maintained at an equilibrium level. Furthermore, the neurotrophin level was increased and pro-inflammatory cytokines were reduced. The evaluation of oxidative stress markers shows that the antioxidants were significantly boosted, and as a result, lipid peroxidation was prevented. The overall findings suggest that SLP can be used as an effective medication for the treatment of sleep disorders like insomnia as it triggers the major neurotransmitter system.