Amyloglucosidase-catalyzed synthesis of n-octyl-D-glucoside--analysis using response surface methodology

• Published in: European food research & technology Vol. 220; no. 3-4; pp. 272 - 277
• Main Authors: Vijayakumar, G.R, Manohar, B, Divakar, S
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.03.2005; Berlin Springer Nature B.V
• Subjects: Biological and medical sciences; Chemical synthesis; equations; Food industries; Food science; Fundamental and applied biological sciences. Psychology; glucan 1,4-alpha-glucosidase; glucose; glucosides; mathematical models; model validation; octanol; optimization; response surface methodology; Studies; synthesis; temperature; yields; Temperature; n-Octanol; Methodology; Octanol; Glucoside; Amyloglucosidase; Buffer volume; n-Octyl-D-glucoside; Synthesis; Volume; Analysis; pH; Response surface
• ISSN: 1438-2377; 1438-2385
• DOI: 10.1007/s00217-004-1057-4

Amyloglucosidase (3.2.1.3)-catalyzed synthesis of n-octyl-d-glucoside was optimized using response surface methodology. A central composite rotatable design involving 32 experiments of five variables at five levels was employed to study the glucosylation reaction. Among the variables studied, namely, n-octanol (15-75 MEq to d-glucose), enzyme (20-100 mg ), pH (4.0-8.0), buffer volume (0.2-1.0 ml) and temperature (30-70°C), amyloglucosidase concentration, pH and temperature were found to be significant. Experimental data fitted the second-order polynomial equation well, as indicated by an R^sup 2^ value of 0.895. Validation experiments carried out under predicted conditions showed good correspondence between experimental and predicted yields. Various surface plots were generated to describe the relationship between operating variables and the conversion yields. The highest yield of 53.5% predicted at optimum conditions of 75 Eq n-octanol, 20 mg amyloglucosidase, 0.2 ml, pH 7.8 buffer at 50 °C showed good correspondence to the experimental yield of 53.8% under these conditions.[PUBLICATION ABSTRACT]