Inhibition of feline immunodeficiency virus gene expression and replication by alphaherpesvirus ICP4 homologues

• Published in: Journal of general virology Vol. 75; no. 10; pp. 2783 - 2787
• Main Authors: Kawaguchi, Yasushi, Maeda, Ken, Miyazawa, Takayuki, Ono, Mitsuru, Tsubota, Kenjiro, Tomonaga, Keizo, Mikami, Takeshi
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.10.1994; Society for General Microbiology
• Subjects: Animals; Base Sequence; Biological and medical sciences; Cats; Cell Line; Chloramphenicol O-Acetyltransferase - analysis; Chloramphenicol O-Acetyltransferase - biosynthesis; feline immunodeficiency virus; Fetus; Fundamental and applied biological sciences. Psychology; Gene Expression; Gene Expression Regulation, Viral; Genetics; herpes simplex virus 1; Herpesvirus 1, Human - genetics; Herpesvirus 1, Human - metabolism; Horses; Immediate-Early Proteins - biosynthesis; Immediate-Early Proteins - metabolism; Immunodeficiency Virus, Feline - genetics; Immunodeficiency Virus, Feline - physiology; Kidney; Microbiology; Molecular Sequence Data; Plasmids; Promoter Regions, Genetic; pseudorabies virus; Repetitive Sequences, Nucleic Acid; Restriction Mapping; Simplexvirus - genetics; Simplexvirus - metabolism; Transcription Factors - metabolism; Transfection; Virology; Virus Replication; Fissipedia; Carnivora; Microorganism interrelationships; Herpesviridae; Alphaherpesvirinae; Retroviridae; Feline immunodeficiency virus; Gene expression; In vitro; Kidney; Virus; Vertebrata; Cell line; Mammalia; Cat; Lentivirinae; Inhibition
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-75-10-2783

Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan We investigated the effects of pseudorabies virus (PRV), herpes simplex virus type 1 (HSV-1), and equineherpesvirus type 1 (EHV-1) ICP4 homologues on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression. This was done by using the transient expression chloramphenicol acetyltransferase (CAT) assay in Crandell feline kidney (CRFK) and Felis catus whole fetus 4 cells transfected with a chimeric FIV LTR-CAT reporter construct in combination with effector plasmids expressing the PRV, HSV-1 or EHV-1 ICP4 homologue. The experiments demonstrated that the ICP4 homologues could significantly inhibit FIV LTR-directed gene expression. Moreover, the ICP4 homologues also exhibited a marked inhibitory effect on FIV replication in CRFK cells cotransfected with an infectious molecular clone of FIV. Received 6 April 1994; accepted 24 May 1994.