High-rate partial nitritation as a pretreatment of anammox process

• Published in: Environmental science and pollution research international Vol. 30; no. 47; pp. 104592 - 104602
• Main Authors: Jin, Yue, Zhang, Xuli, Li, Haixiang, Wu, Zhicheng, Zhang, Wenjie
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2023; Springer Nature B.V
• Subjects: Abundance; Ammonia; Anaerobic Ammonia Oxidation; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Bacteria; Bioreactors - microbiology; Carbon; Community structure; Control theory; Denitrification; Earth and Environmental Science; Ecotoxicology; Effluents; Environment; Environmental Chemistry; Environmental Health; Environmental science; Experiments; Feasibility; Gene expression; Microorganisms; Next-generation sequencing; Nitrification; Nitrogen; Nitrogen - metabolism; Nitrogen dioxide; Nitrosation; Nitrosomonas; Oxidation; Oxidation-Reduction; Reactors; Research Article; Sedimentation & deposition; Sequences; Sewage - microbiology; Sludge; Waste Water Technology; Wastewater; Wastewater treatment; Water Management; Water Pollution Control; Water treatment; Partial nitrification; Hydraulic retention time; High-throughput sequencing; Microbial community
• ISSN: 1614-7499; 0944-1344; 1614-7499
• DOI: 10.1007/s11356-023-29663-7

In this study, a laboratory-scale partial nitrification reactor (PN reactor) was used to treat high-ammonia–nitrogen wastewater, by changing the influent NH 4 + -N conversion rate as the main operating strategy, to investigate the upper limit of its NH 4 + -N conversion rate (ACR) and explore its feasibility as an anammox pre-process. During the experiment, PN reactor was successfully activated in only 10 days. The PN reactor reached the highest ACR value of approximately 10.24 kg N/(m 3 · day) when the influent ACR was 16.57 kg N/(m 3 · day), and the ammonia conversion efficiency (ACE) was 61.78% at this time. The ratio of [NO 2 − -N] Eff /[NH 4 + -N] Eff was approximately 1.37 which was close to the theoretical ratio of 1.32. And feasibility exploration experiment proved that it was feasible to use this PN reactor as a pre-process of anammox. The PCR-DGGE results showed that the dominant phylum and genus in the reactor during the ACR experiment were Proteobacteria and Nitrosomonas , respectively. With the increase in the ACR, the relative concentration of Nitrosomonas sp. G1 increased from 15 to 40%. This indicates that its abundance is directly correlated with the increase in the ACR. High-throughput sequencing showed that increasing the ACR of the PN reactor greatly reduced the diversity and abundance of the system microbial community structure and changed the dominant phylum and genus; however, the stability of the system was not disrupted. High-throughput sequencing experiments showed that the abundance value of nitrosation enzymes accounted for 91.62%, which was positively correlated with the expression of nitrification genes in the genus Nitrosomonas .