CircSSBP2 acts as a MiR-2400 sponge to promote intramuscular preadipocyte proliferation by regulating NDRG1

• Published in: Molecular genetics and genomics : MGG Vol. 299; no. 1; p. 48
• Main Authors: Zhao, Yanqing, Zhang, Wenzhen, Raza, Sayed Haidar Abbas, Qu, Xiaopeng, Yang, Zhimei, Deng, Jiahan, Ma, Jing, Aloufi, Bandar Hamad, Wang, Juze, Zan, Linsen
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2024; Springer Nature B.V
• Subjects: Adipocytes; Adipocytes - cytology; Adipocytes - metabolism; Adipogenesis; Adipogenesis - genetics; Animal Genetics and Genomics; Animal sciences; Animals; Beef; Beef cattle; Biochemistry; Biomedical and Life Sciences; Cattle; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell Proliferation - genetics; Circular RNA; Food quality; Gene Expression Regulation; Genomics; Human Genetics; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; Laboratory animals; Life Sciences; Lipids; Meat quality; Metabolism; Microbial Genetics and Genomics; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; Myc protein; Next-generation sequencing; Original Article; Ovarian cancer; Plant Genetics and Genomics; Preadipocytes; Proteins; RNA, Circular - genetics; Zoology; Proliferation; miR-2400; Bovine; circSSBP2; NDRG1; Intramuscular fat
• ISSN: 1617-4615; 1617-4623
• DOI: 10.1007/s00438-024-02138-1

Intramuscular fat (IMF) is a critical factor in beef quality. IMF is mainly distributed between muscle fibres and its accumulation can affect the marbling and meat quality of beef. IMF formation and deposition is a complex process and in recent years a group of non-coding RNAs (ncRNAs), known as circRNAs, have been discovered to play an important role in regulating intramuscular fat deposition. CircRNAs form a covalent loop structure after reverse splicing of precursor mRNAs. They can act by adsorbing miRNAs, thereby reducing their repressive effects on downstream target genes. Based on high-throughput sequencing of circRNAs in intramuscular fat of Qinchuan and Japanese black cattle, we identified a novel circSSBP2 that is differentially expressed between the two species and associated with adipogenesis. We show that circSSBP2 knockdown promotes bovine intramuscular preadipocyte proliferation, whereas overexpression inhibits bovine intramuscular preadipocyte proliferation. We also show that circSSBP2 can act as a molecular sponge for miR-2400 and that miR-2400 overexpression promotes bovine intramuscular preadipocyte proliferation. Furthermore, N-myc downstream-regulated gene 1 (NDRG1) was identified as a direct target gene of miR-2400, and NDRG1 interference promoted the proliferation of bovine intramuscular preadipocytes. In conclusion, our results suggest that circSSBP2 inhibits the proliferation of bovine intramuscular preadipocytes by regulating the miR-2400/NDRG1 axis.