Construction of an Anoikis-Related Gene Prognostic Signature and Identification of ANGPTL4 as a Key Oncogene in Lung Adenocarcinoma

• Published in: Molecular biotechnology Vol. 66; no. 5; pp. 1290 - 1302
• Main Authors: Lou, Hao, Lin, Xuelian, Wei, Guangyou, Wu, Zelai, Xiao, Youde
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.05.2024; Springer Nature B.V
• Subjects: Adenocarcinoma; Adenocarcinoma of Lung - genetics; Adenocarcinoma of Lung - metabolism; Adenocarcinoma of Lung - pathology; Angiopoietin; Angiopoietin-Like Protein 4 - genetics; Angiopoietin-Like Protein 4 - metabolism; Anoikis; Anoikis - genetics; Apoptosis; Biochemistry; Biological Techniques; Biotechnology; Cancer; Cell Biology; Cell Line, Tumor; Cell migration; Cell Movement - genetics; Chemistry; Chemistry and Materials Science; Cyclin-dependent kinase; Cyclin-dependent kinases; Female; Gene Expression Regulation, Neoplastic; Genes; Human Genetics; Humans; Kinases; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - mortality; Lung Neoplasms - pathology; Lungs; Male; Metastases; Middle Aged; Oncogenes - genetics; Original Paper; Prediction models; Prognosis; Protein Science; Risk; Tumor cells; Tumors; Anoikis; Lung adenocarcinoma; ANGPTL4; Survival prognosis
• ISSN: 1073-6085; 1559-0305
• DOI: 10.1007/s12033-023-01031-3

Anoikis plays an important role in cancer invasion and metastasis. However, the role of anoikis-related genes, AnRGs, in lung adenocarcinoma (LUAD) is not clear. First, anoikis-related genes (AnRGs) were obtained from the Genecard database. Second, the prognostic risk model of AnRGs was established by univariate Cox analysis, the Least Absolute Shrinkage and Selection Operator (LASSO) analysis, and multivariate Cox analysis. Finally, in vitro cell experiments were carried out to determine the expression and function of the key gene AnRGs. Three AnRGs (angiopoietin-like 4, ANGPTL4; Cyclin-Dependent Kinase Inhibitor 3, CDKN3; Solute Carrier Organic Anion Transporter Family Member 1B3, SLCO1B3) were screened for the construction of risk prediction model. Additionally, ANGPTL4 was significantly highly expressed in tumor cells, and the knockdown of ANGPTL4 expression on tumor cells could inhibit tumor cell migration and apoptosis. Constructing a risk model based on anoikis-related genes can effectively differentiate the prognosis of LUAD. ANGPTL4 can be used as a potential new target for LUAD treatment.