The role of galectin-3 in patients with permanent and paroxysmal Atrial Fibrillation and echocardiographic parameters of left atrial fibrosis

• Published in: Molecular biology reports Vol. 50; no. 11; pp. 9019 - 9027
• Main Authors: Mariana Barros Melo da Silveira, Maria, Victor Batista Cabral, João, Tavares Xavier, Amanda, Palmeira do Ó, Kleyton, Francisco de Moura Junior, Julio, Tavares de Carvalho, Osmário, Bezerra Mendes Filho, Edivaldo, Furtado de Mendonça Belmont, Taciana, Maria Del Castillo, José, Jesus Barreto de Melo Rêgo, Moacyr, Richardson da Silva Vasconcelos, Luydson, Celestino Sobral Filho, Dário, Cavalcanti de Oliveira, Dinaldo
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.11.2023; Springer Nature B.V
• Subjects: Aged; Animal Anatomy; Animal Biochemistry; Atrial Fibrillation - diagnostic imaging; Atrial Fibrillation - genetics; Biochemical markers; Biomedical and Life Sciences; Cardiac arrhythmia; Clinical medicine; Echocardiography; Echocardiography - methods; Electrocardiography; Emergency medical care; Enzymes; Fibrosis; Galectin 3 - genetics; Galectin-3; Gene polymorphism; Genetic testing; Haplotypes; Heart Atria - diagnostic imaging; Heart Atria - pathology; Histology; Humans; Life Sciences; Middle Aged; Molecular biology; Morphology; Original Article; Patients; Polymorphism; Serum levels; Single-nucleotide polymorphism; Software; Ultrasonic imaging; Speckle-tracking echocardiography; Galectin-3; Atrial fibrosis; Atrial fibrillation; Genetic polymorphism
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-023-08774-x

Background Biochemical markers and imaging tests have been used with the aim of stratifying the risk and detecting atrial fibrosis. Speckle-tracking echocardiography (STE) is used for the detection of atrial fibrosis and Gal-3 provides an important prognostic value. The objective of the study was to assess the association between atrial fibrosis markers and serum levels, genetic polymorphisms and genic expression of galectin-3. Methods 206 patients with permanent AF and 70 patients with paroxysmal AF were included in the study. Real time PCR (TaqMan) system was used to study SNPs rs4652 and 4644 of the gene LGALS3 . Serum levels of Gal-3 were determined by ELISA and STE was performed to assess fibrosis. Results Mean age of individuals with permanent AF was 66.56 ± 12 years. As for the echocardiography results, those patients showed an decrease in the following parameters peak atrial longitudinal strain (PALS) (p = 0.002) when compared to the same parameters from the paroxysmal AF group of patients. There was a correlation between serum levels of Gal-3 and PALS in the group of patients with permanent AF; the lower the levels of gal-3, the lower the LA strain (r = 0.24; p = 0.01). Conclusions Echocardiographic findings showed association with the groups, and with serum levels of Gal-3 in patients with permanent AF. The distribution of allelic and genotypic frequencies, and of the haplotypes of polymorphism LGALS3 rs4652 and rs4644 did not present statistical variation, which suggests that those SNPs are not associated with the AF clinical forms (permanent and paroxysmal).