Nitric oxide-releasing albumin nanoclusters facilitate healing of methicillin-resistant Staphylococcus aureus-infected cutaneous wounds

Purpose Although nitric oxide (NO)-releasing nanoparticles have garnered significant attention owing to their potent antibacterial effects against methicillin-resistant Staphylococcus aureus (MRSA) and direct promotion of wound healing, the majority of NO-releasing nanoparticles are composed of synt...

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Bibliographic Details
Published inJournal of pharmaceutical investigation Vol. 54; no. 1; pp. 51 - 60
Main Authors Kwak, Dongmin, Lee, Juho, Kim, Jihyun, Kim, Hyunwoo, Lee, Jae-Young, Kim, Dae-Duk, Yoo, Jin-Wook
Format Journal Article
LanguageEnglish
Published Singapore Springer Nature Singapore 01.01.2024
한국약제학회
Subjects
Biomedical and Life Sciences
Biomedicine
Original Article
약학
Albumin
Wound healing
Methicillin-resistant
Nitric oxide
Nanoclusters
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ISSN2093-5552
2093-6214
DOI10.1007/s40005-023-00641-y

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Summary:Purpose Although nitric oxide (NO)-releasing nanoparticles have garnered significant attention owing to their potent antibacterial effects against methicillin-resistant Staphylococcus aureus (MRSA) and direct promotion of wound healing, the majority of NO-releasing nanoparticles are composed of synthetic polymers or inorganic materials, which may lead to unfavorable effects in the body. To overcome this limitation, we developed NO-releasing albumin nanoclusters (NO/ANCs) using bovine serum albumin, a fully biocompatible endogenous material. Methods NO/ANCs were fabricated by incorporating diethylenetriamine diazeniumdiolate (DT/NO) into albumin nanoclusters via electrostatic interactions, followed by glutaraldehyde crosslinking between the albumin molecules. The antibacterial effects of the NO/ANCs were investigated using the LIVE/DEAD® BacLight™ bacterial viability kit. The enhanced wound healing and in vivo antibacterial effects were evaluated in a mouse model of MRSA-challenged full-thickness wounds. Results NO/ANCs were successfully fabricated and could release NO for more than 17 h. NO/ANCs demonstrated enhanced in vitro antibacterial effects and exhibited improved in vivo antibacterial and wound-healing effects in a mouse model of MRSA-challenged full-thickness wounds. Conclusion NO/ANCs could be a promising formulation for the treatment of MRSA-infected cutaneous wounds.
ISSN:2093-5552
2093-6214
DOI:10.1007/s40005-023-00641-y