Polymorphisms in nanog are associated with oral leukoplakia: case–control study

• Published in: Odontology Vol. 113; no. 3; pp. 1263 - 1268
• Main Authors: Michels, Arieli Carini, Scheifer, Suelen Teixeira, Tiboni, Fernanda, Modolo, Filipe, dos Santos, Emanuela Carla, Scariot, Rafaela, Aguiar, Maria Cassia Ferreira, Ignácio, Sergio Aparecido, de Noronha, Lucia, Marins, Mariana Hornung, Jham, Bruno Correia, de Souza, Cleber Machado, Johann, Aline Cristina Batista Rodrigues
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.07.2025; Springer Nature B.V
• Subjects: Adult; Alleles; Case-Control Studies; Dentistry; Female; Genetic Predisposition to Disease; Genotype; Genotyping; Homeodomain Proteins - genetics; Humans; Leukokeratosis; Leukoplakia, Oral - genetics; Male; Medicine; Middle Aged; Nanog Homeobox Protein - genetics; Oral and Maxillofacial Surgery; Original Article; Polymerase chain reaction; Polymorphism, Single Nucleotide; Real-Time Polymerase Chain Reaction; Statistical analysis; Nanog homeobox protein; Embryonic stem cells; Oral leukoplakia; Genetic polymorphism
• ISSN: 1618-1247; 1618-1255; 1618-1255
• DOI: 10.1007/s10266-024-01044-7

To investigate the association of NANOG polymorphisms with oral leukoplakia. In this case–control study, 68 cases of oral leukoplakia, and 21 of normal oral mucosa (control) were submitted to genotyping of tagSNPs polymorphisms: rs877716 and rs10845877 in NANOG , through real-time polymerase chain reaction (PCR). Pearson’s chi-squared and Fisher’s exact statistical tests were used, with a significance of 5%. For the dominant genetic model of the G allele of rs877716, the genotypes AG + GG revealed higher frequency in subjects in the oral leukoplakia group than in control subjects (75,4 and 50% respectively; p = 0,031). Subjects with this genotype were 3,063 times more likely to develop oral leukoplakia compared to subjects with AA. In the allelic genetic model, for rs10845877, the C allele was more frequent in subjects with leukoplakia than in control subjects (25 and 7, 5% respectively; p = 0, 01). There was no association found in the other genetic models. Polymorphisms in NANOG are associated with oral leukoplakia.