Intravenous Administration of Heterodimeric Phospholipase A2 HDP-1 Disturbs Cardiovascular System Functions and Biochemical Composition of Blood in Anesthetized Rats

• Published in: Doklady. Biochemistry and biophysics Vol. 522; no. 1; pp. 273 - 278
• Main Authors: Ismailova, A. M., Severyukhina, M. S., Shaykhutdinova, E. R., Perepechenova, N. A., Starkov, V. G., Dyachenko, I. A., Tsetlin, V. I., Utkin, Yu. N.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.06.2025; Springer Nature B.V
• Subjects: Administration, Intravenous; Anesthesia; Animals; Arrhythmia; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Blood Pressure - drug effects; Cardiovascular system; EKG; Electrocardiography; Innervation; Intravenous administration; Life Sciences; Male; Molecular Biology; Phospholipase; Phospholipase A2; Phospholipases A2 - administration & dosage; Phospholipases A2 - chemistry; Phospholipases A2 - pharmacology; Rats; Rats, Wistar; Venom; Viper Venoms - administration & dosage; blood pressure; heart rate; hemodynamic parameters; arrhythmia; phospholipase A2; biochemical blood parameters; SD rats
• ISSN: 1607-6729; 1608-3091; 1608-3091
• DOI: 10.1134/S1607672925600137

Heterodimeric phospholipase A2 HDP-1 from the venom of Nikolsky viper consists of two noncovalently bound subunits—HDP-1P and HDP-1In, the first of which exhibits lipolytic activity, while the second does not. The subunits have similar molecular masses and amino acid sequences. Administration of HDP-1 and HDP-1P to anesthetized rats is accompanied by prolonged hypotensive effects, the intensity of which depends on the dose. After the administration of HDP-1P, the effect develops more slowly than in the case of HDP-1, containing both subunits, while individual HDP-1In does not affect hemodynamic parameters. It was found using electrocardiography that HDP-1 and HDP-1P affect the functioning of the rat heart, causing arrhythmia, which may indicate their ability to affect the innervation of the heart. The administration of HDP-1 and its subunits also leads to changes in blood biochemical parameters, indicating their damaging effect on cells and internal organs.