The effect of tannic acid on renal renin-angiotensin signaling pathway in a model of unilateral ureteral obstruction in male Wistar rats

• Published in: Naunyn-Schmiedeberg's archives of pharmacology Vol. 396; no. 12; pp. 3569 - 3579
• Main Authors: Kianfar, Tina, Kadkhodaee, Mehri, Seifi, Behjat, Abdi, Arash, Adelipour, Maryam, Pishkenari, Bahar Hejazi, Malboosi, Nasrin, Ranjbaran, Mina
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2023; Springer Nature B.V
• Subjects: Acids; Angiotensin; Angiotensin II; Angiotensinogen; Angiotensinogen - metabolism; Angiotensinogen - pharmacology; Animal models; Animals; Biomedical and Life Sciences; Biomedicine; Creatinine; Endocrine system; Fibrosis; Inflammation; Kidney; Kidney diseases; Male; Nephrectomy; Neurosciences; Oxidative stress; Pharmacology/Toxicology; Protein folding; Rats; Rats, Wistar; Renal function; Renin; Renin - genetics; Signal Transduction; Superoxide dismutase; Tannic acid; Tumor necrosis factor-α; Ureter; Ureteral Obstruction - complications; Ureteral Obstruction - drug therapy; Tannic acid; Ureteral obstruction; Oxidative stress; Inflammation; Renin-angiotensin system
• ISSN: 0028-1298; 1432-1912
• DOI: 10.1007/s00210-023-02548-9

The purpose of current study was to elucidate polyphenol tannic acid effect on renal function and activity of the renin-angiotensin system after unilateral ureteral obstruction (UUO). Male Wistar rats were divided into three groups of six randomly: 1) Sham, 2) UUO, and 3) UUO + Tannic acid. Rats in the UUO and UUO + Tannic acid groups experienced unilateral ureteral obstruction. In the Sham group, the abdominal cavity was exposed without UUO induction. In the UUO + Tannic acid group, animals received tannic acid (20 mg/kg) intraperitoneally, 6 and 12 h after clamping the left ureter and 6 and 12 h after the right nephrectomy. Blood samples were taken to measure blood urea nitrogen (BUN) and creatinine levels. Kidney tissue samples were obtained for assessment of oxidative stress, inflammatory indices and the levels of renin-angiotensin system components. Tannic acid administration significantly improved UUO-induced kidney dysfunction (serum BUN: 66.42 ± 14.414 mg/dl, p  < 0.05; serum creatinine: 1.67 ± 0.258 mg/dl, p  < 0.05), oxidative stress (MDA level: 95.29 ± 37.35 µmol/g tissue, p  < 0.05; SOD activity: 59.82 ± 13.41 U/g protein, p  < 0.01) and inflammation (renal TNF-α: 57.05 ± 15.653 pg/g tissue, p  < 0.05; renal IL-6: 117.015 ± 24.076 pg/g tissue, p  < 0.001). The treatment caused a reduction in the amount of renal angiotensinogen, renin and ACE genes expression compared to the UUO group (Angiotensinogen: 8.9 ± onefold, p  < 0.05, Renin: 6.5 ± 1.14 fold, p  < 0.05, ACE: 4.9 ± 0.64 fold, p  < 0.05). Angiotensin II type 1 receptor protein levels decreased in the tannic acid-treated rats in comparison with the UUO group (0.61 ± 0.136, p  < 0.05). According to the result of the current study, tannic acid considerably attenuated the complications of unilateral ureteral obstruction through renin-angiotensin system modulation. Trial registration: IR.TUMS.MEDICINE.REC.1400.802. Graphical Abstract