Effects of Sr2+, BO33−, and SiO32− on Differentiation of Human Dental Pulp Stem Cells into Odontoblast-Like Cells

• Published in: Biological trace element research Vol. 201; no. 12; pp. 5585 - 5600
• Main Authors: Miyano, Yuko, Mikami, Masato, Katsuragi, Hiroaki, Shinkai, Koichi
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.12.2023; Springer Nature B.V
• Subjects: Alizarin; Alkaline phosphatase; Biochemistry; Biomedical and Life Sciences; Biotechnology; Calcification; Cell culture; Cell differentiation; Cell proliferation; Culture; Culture media; Dental pulp; Dentin; Differentiation; Dspp protein; Gene expression; Immunocytochemistry; Ions; Life Sciences; Nestin; Nucleotide sequence; Nutrition; Oncology; PCR; Phosphatase; Polymerase chain reaction; Proliferation; Silicates; Stem cells; Strontium; Substrates; Strontium; Human dental pulp stem cells; Silicate; S-PRG; Borate; Odontogenic differentiation
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-023-03625-z

This study aimed to clarify the effects of strontium (Sr 2+ ), borate (BO 3 3− ), and silicate (SiO 3 2− ) on cell proliferative capacity, the induction of differentiation into odontoblast-like cells (OLCs), and substrate formation of human dental pulp stem cells (hDPSCs). Sr 2+ , BO 3 3− , and SiO 3 2− solutions were added to the hDPSC culture medium at three different concentrations, totaling nine experimental groups. The effects of these ions on hDPSC proliferation, calcification, and collagen formation after 14, 21, and 28 days of culture were evaluated using a cell proliferation assay, a quantitative alkaline phosphatase (ALP) activity assay, and Alizarin Red S and Sirius Red staining, respectively. Furthermore, the effects of these ions on hDPSC differentiation into OLCs were assessed via quantitative polymerase chain reaction and immunocytochemistry. Sr 2+ and SiO 3 2− increased the expression of odontoblast markers; i.e., nestin, dentin matrix protein-1, dentin sialophosphoprotein, and ALP genes, compared with the control group. BO 3 3− increased the ALP gene expression and activity. The results of this study suggested that Sr 2+ , BO 3 3− , and SiO 3 2− may induce hDPSC differentiation into OLCs.