Detection of proteolytic enzymes in agar electrophoresis

An agar substrate containing hyaluronic acid and horse serum for the detection of hyaluronidase activity following electrophoresis was found suitable for the detection of proteolysis. The substrate was refined for the detection of only proteolysis by elimination of the hyaluronic acid and substituti...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 53; no. 2; pp. 514 - 521
Main Authors Kenneth Herd, J., Motycka, Lyle
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.01.1973
Subjects
Animals
Cattle
Electrophoresis
Horses
Hyaluronic Acid
Hyaluronoglucosaminidase - analysis
Male
Methods
Papain - analysis
Peptide Hydrolases - analysis
Pronase - analysis
Serum Albumin, Bovine
Testis - enzymology
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Summary:An agar substrate containing hyaluronic acid and horse serum for the detection of hyaluronidase activity following electrophoresis was found suitable for the detection of proteolysis. The substrate was refined for the detection of only proteolysis by elimination of the hyaluronic acid and substitution of bovine serum albumin in place of the serum. The refined substrate revealed two bands of proteolytic activity in papain. The refined substrate also revealed weak proteolytic activity in a “chromatographically pure” preparation of hyaluronidase. The method should be adaptable for the detection of multiple electrophoretic forms of several proteolytic enzymes.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(73)90101-2