An evaluation of photobiomodulation effects on human gingival fibroblast cells under hyperglycemic condition: an in vitro study

• Published in: Lasers in medical science Vol. 39; no. 1; p. 9
• Main Authors: Iranpour, Babak, Mohammadi, Kimia, Hodjat, Mahshid, Hakimiha, Neda, Sayar, Ferena, Kharazi Fard, Mohammad Javad, Sadatmansouri, Saeed, Hanna, Reem
• Format: Journal Article
• Language: English
• Published: London Springer London 19.12.2023; Springer Nature B.V
• Subjects: Cell growth; Cell proliferation; Dentistry; Evaluation; Fibroblasts; Fluorimetry; Gene expression; Glucose; Growth factors; Hyperglycemia; Inflammation; Inflammatory response; Interleukin 6; Irradiation; Lasers; Light therapy; Medicine; Medicine & Public Health; Optical Devices; Optics; Original Article; Oxygen; Photonics; Polymerase chain reaction; Post-irradiation; Quantum Optics; Reactive oxygen species; Semiconductor lasers; Vascular endothelial growth factor; Fibroblasts; Oxidative stress; Hyperglycemia; Reactive oxygen species; Vascular endothelial growth factor; Photobiomodulation
• ISSN: 1435-604X; 0268-8921; 1435-604X
• DOI: 10.1007/s10103-023-03954-4

An in vitro study was designed to evaluate the effects of photobiomodulation (PBM) with 915-nm diode laser on human gingival fibroblast (HGF) cells under hyperglycemic condition. The HGF cells were cultured in Dulbecco’s modified eagle medium (DMEM) medium containing 30 mM glucose concentration for 48 h to mimic the hyperglycemic condition. Subsequently, the cells received three sessions of PBM (915 nm, continuous emission mode, 200 mW, energy density values of 3.2, 6, and 9.2 J/cm 2 ). Twenty-four hours post-irradiation, cell proliferation, expression of interleukin 6 (IL-6), and vascular endothelial growth factor (VEGF) were assessed with MTT and real-time polymerase chain reaction (PCR) tests, respectively. Also, reactive oxygen species (ROS) production was measured using CM-H2DCFDA fluorimetry. No changes were detected in the cell proliferation rate between the high glucose control group and laser-treated cells, while VEGF and IL-6 gene expression levels increased significantly after PBM in the high glucose-treated cells group. ROS level was significantly decreased in the irradiated cells in high-glucose medium compared with the high glucose control group. Our study revealed the inductive role of 915-nm–mediated PBM on VEGF and the inflammatory response while concurrently reducing reactive oxygen species production in HGF cells in hyperglycemic conditions.