Identification of bacterial community in a rapid composting method using 16SrDNA genes sequencing

• Published in: Biodegradation (Dordrecht) Vol. 35; no. 4; pp. 361 - 370
• Main Authors: Shahid, Muhammad, Ahmed, Waqar, Khan, Abdul Basit, Shams, Zafar Iqbal, Arif, Afsheen
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.07.2024; Springer Nature B.V
• Subjects: Aquatic Pollution; Bacteria - genetics; Bacteria - isolation & purification; Biodegradation; Biodegradation, Environmental; Biomedical and Life Sciences; Citrobacter; Composting; Consortia; DNA, Bacterial - genetics; E coli; Gene sequencing; Geochemistry; Human resources; Klebsiella; Life Sciences; Microbial Consortia - genetics; Microbial degradation; Microbiology; Microorganisms; Municipal solid waste; Municipal waste management; Nucleotide sequence; Organic wastes; Original Paper; PCR; Phylogeny; Refuse Disposal - methods; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequencing; Soil Microbiology; Soil Science & Conservation; Solid Waste; Solid waste management; Solid wastes; Terrestrial Pollution; Waste management; Waste Management/Waste Technology; Waste Water Technology; Water Management; Water Pollution Control; Bacterial consortia; Municipal waste management; Bio composting
• ISSN: 0923-9820; 1572-9729; 1572-9729
• DOI: 10.1007/s10532-023-10056-z

Composting is a process of microbial degradation of organic waste and is commonly applied for waste management. This is a slow process and requires a lot of land and human resources. The present study investigated mechanical augmentation with required microbial culture for composting municipal solid waste (MSW). Thirty isolates were subjected to 16S rDNA PCR amplification and gene sequencing. The isolates' sequencing from the compost samples was processed on BLASTn. Fourteen strains were identified for further experiments. The results divulge that Empedobacter (04), Bacillus (02), Proteus (02), Lactiplantibacillus (01), Klebsiella (01), Citrobacter (01), Brevibacillus (01), E. coli (01) and one unidentified strain were growing during composting. Eleven combinations of bacterial consortium and respective additives were applied for the organic waste decomposition in the next stage, resulting in varied completion periods ranging from 3 to 14 days. Two combinations were completed within 3 days, which are considered ideal combinations for composting. The microbial consortium was significantly diverse, which is a reason for rapid biodegradation. The present study reveals that the technology will be highly feasible for municipal solid waste management in tropical/subtropical countries.