Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus in Xinjiang, China, from 2020 to 2022

• Published in: Archives of virology Vol. 169; no. 5; p. 96
• Main Authors: Chen, Jinlong, Tian, Lulu, Liu, Yucheng, Sun, Yaoqiang, Li, Zhiyuan, Cai, Xuepeng, Meng, Qingling, Qiao, Jun
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.05.2024; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Diarrhea; Epidemics; Genetic analysis; Genetic diversity; Genomes; Genotype & phenotype; Genotypes; Infectious Diseases; Medical Microbiology; Nucleotide sequence; Original Article; Phylogenetics; Phylogeny; Polymerase chain reaction; Recombination; S gene; S1 gene; Strains (organisms); Transmissible gastroenteritis; Vaccines; Virology; China
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-024-06029-z

In recent years, the pig industry in Xinjiang, China, has been severely impacted by outbreaks of porcine epidemic diarrhea (PED), despite vaccination efforts. In this study, we investigated the genetic characteristics of currently prevalent porcine epidemic diarrhea virus (PEDV) strains in the region. We collected 548 samples from animals with suspected PED on large-scale pig farms in Xinjiang. Of these, 258 tested positive for PEDV by RT-PCR, yielding an overall positivity rate of 47.08%. S1 gene sequencing and phylogenetic analysis were conducted on 23 randomly selected RT-PCR-positive samples. Three endemic strains of PEDV (PEDV/CH/XU/2020, PEDV/CH/XK/2020, and PEDV/CH/XA/2020) were isolated, and their complete genome sequences were analyzed for evidence of genetic recombination. Sequence comparison of the S gene indicated significant variations in the S1 gene of the Xinjiang strains compared to the vaccine strains CV777, AJ1102, and LWL, with 90.2%-98.5% nucleotide sequence identity. Notably, both the N-terminal and C-terminal domains of the S protein showed significant variation. Genetic evolutionary analysis identified the GIIa subtype as the dominant genotype among the epidemic strains in Xinjiang. Recombination analysis revealed inter-subtype recombination events in the PEDV/CH/XK/2020 and XJ1904-34 strains. These findings highlight the extensive genetic variation in the predominant GIIa genotype of PEDV in Xinjiang, which does not match the genotype of the currently used vaccine strains. These data may guide further efforts toward the development of effective vaccines for the control of PED.