Changes in pituitary gonadotropin subunits and hypothalamic Kiss-1 gene expression by administration of sex steroids in ovary-intact female rats

• Published in: Endocrine Vol. 83; no. 3; pp. 733 - 746
• Main Authors: Yacca, Susdiaman S., Kanasaki, Haruhiko, Tumurbaatar, Tuvshintugs, Cairang, Zhuoma, Oride, Aki, Okada, Hiroe, Kyo, Satoru
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2024; Springer Nature B.V
• Subjects: 17β-Estradiol; Arcuate nucleus; Diabetes; Dihydrotestosterone; Endocrinology; Estrus cycle; Follicle-stimulating hormone; Gene expression; Gonadotropin-releasing hormone; Gonadotropins; Humanities and Social Sciences; Hypothalamus; Hypothalamus (anterior); Internal Medicine; Kiss1 protein; Luteinizing hormone; Medicine; Medicine & Public Health; multidisciplinary; Original Article; Ovaries; Periventricular nucleus; Pituitary; Pituitary (anterior); Pituitary gland; Preoptic area; Progesterone; Science; Steroid hormones; Steroids; Kisspeptin; Gonadotropins; Hypothalamus; Sex steroids; Pituitary
• ISSN: 1559-0100; 1355-008X; 1559-0100
• DOI: 10.1007/s12020-023-03596-0

Objective We examined how the sex steroids influence the synthesis of gonadotropins. Materials and Methods The effects of sex steroids estradiol (E2), progesterone (P4), and dihydrotestosterone (DHT) in pituitary gonadotroph cell model (LβT2 cells) in vitro and ovary-intact rats in vivo were examined. The effects of sex steroids on Kiss1 gene expression in the hypothalamus were also examined in ovary-intact rats. Results In LβT2 cells, E2 increased common glycoprotein alpha (Cga) and luteinizing hormone beta (Lhb) subunit promoter activity as well as their mRNA expression. Although gonadotropin subunit promoter activity was not modulated by P4, Cga and Lhb mRNA expression was increased by P4. DHT inhibited Cga and Lhb mRNA expression with a concomitant decrease in their promoter activity. During the 2-week administration of exogenous E2 to ovary-intact rats, the estrous cycle determined by vaginal smears was disrupted. P4 or DHT administration completely eliminated the estrous cycle. Protein expression of all three gonadotropin subunits within the pituitary gland was inhibited by E2 or P4 treatment in vivo; however, DHT reduced Cga expression but did not modulate Lhb or follicle-stimulating hormone beta subunit expression. E2 administration significantly repressed Kiss1 mRNA expression in a posterior hypothalamic region that included the arcuate nucleus. P4 and DHT did not modulate Kiss1 mRNA expression in this region. In contrast, P4 administration significantly inhibited Kiss1 mRNA expression in the anterior region of the hypothalamus that included the anteroventral periventricular nucleus. The expression of gonadotropin-releasing hormone (Gnrh) mRNA in the anterior hypothalamic region, where the preoptic area is located, appeared to be decreased by treatment with E2 and P4. Conclusion Our findings suggest that sex steroids have different effects in the hypothalamus and pituitary gland.