Viable Escherichia coli enumeration on a polydimethylsiloxane (PDMS) chip with vertical channel-well configuration
The culture-based methods for viable Escherichia coli ( E. coli ) detection suffer from long detection time and laborious procedures, whereas the molecule tests and immune recognition technologies lack live/dead E. coli differentiation. Rapid, easy-to-use, and accessible viable E. coli detection is...
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Published in | Mikrochimica acta (1966) Vol. 191; no. 5; p. 241 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Vienna
Springer Vienna
01.05.2024
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The culture-based methods for viable
Escherichia coli
(
E. coli
) detection suffer from long detection time and laborious procedures, whereas the molecule tests and immune recognition technologies lack live/dead
E. coli
differentiation. Rapid, easy-to-use, and accessible viable
E. coli
detection is of benefit to bacterial infection diagnosis and risk warning of
E. coli
contamination of water and food, safeguarding human health. Herein, we propose a microwell chip-based solution to realize simple and rapid determination of viable
E. coli.
The vertical channel-well configuration is applied to develop the microwell array chip for increasing the microwell density (6200 wells/cm
2
), yielding a broad dynamic range from 10
3
to 10
7
CFU/mL. We incorporate an inducible enzyme assay with the developed chip and achieve the differentiation of live/dead
E. coli
within 4 h, significantly shortening the detection time from over 24 h in the standard method. By encapsulating single
E. coli
into microwells, the concentration of viable cells can be determined simultaneously through counting positive microwells. In addition, the air soluble PDMS that can store negative pressure for independent sample digitalization endows the developed chip with simple operation and less reliance on external equipment. With further developments for increasing the number of microwell and integrating more sample panels, the developed chip can become a useful tool for rapid viable
E. coli
enumeration with user-friendly operation, simple procedures, and accessibility in decentralized settings, thereby deploying this device for water and food safety monitoring, as well as clinical bacterial infection diagnosis.
Graphical Abstract |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0026-3672 1436-5073 |
DOI: | 10.1007/s00604-024-06338-9 |