HIV-1 genotypic resistance profile of patients failing antiretroviral therapy in Paraná, Brazil

Antiretroviral therapy (ART) has reduced morbidity and mortality related to human immunodeficiency virus (HIV) infection, but in spite of this advance, HIV mutations decrease antiretroviral susceptibility, thus contributing to treatment failure in patients. Genotyping HIV-1 allows the selection of n...

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Published inThe Brazilian journal of infectious diseases Vol. 14; no. 4; pp. 360 - 371
Main Authors Toledo, Paula Virginia Michelon, Carvalho, Denise Siqueira de, Romagnoli, Luiza, Marcinko, Gustavo, Cunha, Clovis Arns da, Souza, Margely Nunes de, Brindeiro, Rodrigo, Queiroz-Telles, Flávio de
Format Journal Article
LanguageEnglish
Published Brazilian Society of Infectious Diseases 01.08.2010
Elsevier
Subjects
antiretrovirals
drug-experienced patients
genotype
HIV-1
INFECTIOUS DISEASES
HIV-1
drug-experienced patients
antiretrovirals
genotype
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Summary:Antiretroviral therapy (ART) has reduced morbidity and mortality related to human immunodeficiency virus (HIV) infection, but in spite of this advance, HIV mutations decrease antiretroviral susceptibility, thus contributing to treatment failure in patients. Genotyping HIV-1 allows the selection of new drugs after initial drug failure. This study evaluated the genotypic profile of HIV-1 isolates from treated (drug-experienced) patients in Paraná, Brazil. The prevalence of mutations in reverse transcriptase (RT) and protease (PR) genes were assessed. We analyzed 467 genotypes of patients with HIV-1 viral loads above 1,000 copies/mL. Mutations at HIV-1 RT and PR genes and previously used ART regimens were recorded. The most prevalent RT mutations were: 184V (68.31%), 215YF (51.6%), 103NS (46%), 41L (39.4%), 67N (38.54%), 210W (23.5%), 190ASE (23.2%), and 181C (17.4%). PR mutations were 90M (33.33%), 82ATFS (29%), 46I (26.8%) and 54V (22.2%). The prevalence of mutations was in line with previous national and international reports, except to nonnucleoside analogue reverse transcriptase inhibitors related mutations, which were more prevalent in this study. Previous exposure to antiretroviral drugs was associated with genotypic resistance to specific drugs, leading to treatment failure in HIV patients.
ISSN:1413-8670
1678-4391
DOI:10.1590/S1413-86702010000400009