Detection of Epidermal Growth Factor Receptor (EGFR) Gene Mutation in Formalin Fixed Paraffin Embedded Tissue by Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) in Non-Small Cell Lung Cancer in the Northeastern Region of Thailand

• Published in: Asian Pacific journal of cancer prevention : APJCP Vol. 20; no. 5; pp. 1339 - 1343
• Main Authors: Saiyaros, Kornsiri, Kritpetcharat, Panutas, Pairojkul, Chawalit, Sithithaworn, Jiraporn
• Format: Journal Article
• Language: English
• Published: Thailand West Asia Organization for Cancer Prevention 25.05.2019
• Subjects: Adenocarcinoma - epidemiology; Adenocarcinoma - genetics; Adenocarcinoma - pathology; Adenocarcinoma, Bronchiolo-Alveolar - epidemiology; Adenocarcinoma, Bronchiolo-Alveolar - genetics; Adenocarcinoma, Bronchiolo-Alveolar - pathology; Adult; Aged; Base Sequence; Biomarkers, Tumor - genetics; Carcinoma, Non-Small-Cell Lung - epidemiology; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - pathology; Carcinoma, Squamous Cell - epidemiology; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; Case-Control Studies; ErbB Receptors - genetics; Female; Follow-Up Studies; Formaldehyde - chemistry; Humans; Lung Neoplasms - epidemiology; Lung Neoplasms - genetics; Lung Neoplasms - pathology; Male; Middle Aged; Mutation; Paraffin Embedding - methods; Polymerase Chain Reaction - methods; Polymorphism, Single-Stranded Conformational; Prognosis; Sequence Homology; Thailand - epidemiology; Thailand; single strand conformational polymorphism; Epidermal growth factor receptor; polymerase chain reaction
• ISSN: 1513-7368; 2476-762X
• DOI: 10.31557/APJCP.2019.20.5.1339

Background: The use of targeted specific genes in therapeutic and treatment decisions has been considered for lung cancer. The epidermal growth factor receptor (EGFR) gene, which is over expressed in non-small cell lung cancer (NSCLC), was considered as one of the targeted specific genes. EGFR mutations in exons 18–21, which encode a portion of the EGFR kinase domain, were found in NSCLC patients and were associated with the response of EGFRtyrosine kinase inhibitors (EGFR-TKIs). Therefore, a molecular technique for EGFR mutation detection has important benefits for therapy in NSCLC patients. This study aims to determine the EGFR mutations in patients with NSCLC using polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) in exons 18-21. Methods: DNA samples were extracted from formalin fixed paraffin embedded tissues of NSCLC patients who attended hospital. The extracted DNA was used as a template for the EGFR gene amplification. Results: Occurrence of EGFR mutations were found in 29 out of 50 cases (58%).The frequency of EGFR mutations by first PCR at exon 18, 19, 20 and 21 were 6 (12%), 19 (38%) 20 (40%) and at 21 (42%), respectively. By PCR-SSCP, the frequencies of EGFR mutations at exon 18, 19, 20 and 21 were 3(6%), 18(36%), 23(46%) and 13(26%), respectively. All of the mutations found were in agreement with DNA sequencings. Conclusion: The high frequency of EGFR mutations in NSCLC suggests that PCR-SSCP is a efficient screening method and useful for treatment plan.