Decreased serum dependence in the growth of NIH3T3 cells from the overexpression of human nuclear receptor-binding SET-domain-containing protein 1 (NSD1) or fission yeast su(var)3-9, enhancer-of-zeste, trithorax 2 (SET2)

• Published in: Cell biochemistry and function Vol. 26; no. 2; pp. 146 - 150
• Main Authors: Yamada-Okabe, Toshiko, Matsumoto, Naomichi
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.03.2008
• Subjects: Amino Acid Sequence; Animals; Cell Proliferation; Culture Media - chemistry; Gene Expression Profiling; histone H3K36; Histone-Lysine N-Methyltransferase - biosynthesis; Histone-Lysine N-Methyltransferase - genetics; Histone-Lysine N-Methyltransferase - physiology; Humans; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - physiology; Mice; NIH 3T3 Cells; NSD1; Nuclear Proteins - biosynthesis; Nuclear Proteins - genetics; Nuclear Proteins - physiology; Protein Conformation; Protein Structure, Tertiary; Reverse Transcriptase Polymerase Chain Reaction; Schizosaccharomyces - metabolism; Schizosaccharomyces pombe Proteins - biosynthesis; Schizosaccharomyces pombe Proteins - genetics; Schizosaccharomyces pombe Proteins - physiology; Sequence Homology, Amino Acid; serum; SET domain; SET2
• ISSN: 0263-6484; 1099-0844
• DOI: 10.1002/cbf.1413

Nuclear receptor‐binding SET‐domain‐containing protein 1 (NSD1), a culprit gene for Sotos syndrome, contains a su(var)3‐9, enhancer‐of‐zeste, trithorax (SET) domain that is responsible for histone methyltransferase activity and other domains such as plant homeodomain (PHD) and proline‐tryptophan‐tryptophan‐proline (PWWP) involved in protein–protein interactions in the C‐terminal half of NSD1. To elucidate the function of NSD1 on cell growth, we overexpressed NSD1 in NIH3T3 cells. Cells overexpressing NSD1 grew in the presence of 2% serum, whereas vector transfected cells did not. Overexpression of the C‐terminal half of NSD1 but not the N‐terminal half of NSD1 also produced cell growth under low serum concentration. Furthermore, overexpression in NIH3T3 of Schizosaccharomyces pombe SET2 which has a SET domain but not PHD or PWWP domains conferred the reduced serum dependence. Thus, the SET domain of NSD1 is involved in cell growth by modulating serum dependence. Copyright © 2007 John Wiley & Sons, Ltd.