miR-140 and miR-196a as Potential Biomarkers in Breast Cancer Patients

• Published in: Asian Pacific journal of cancer prevention : APJCP Vol. 21; no. 7; pp. 1913 - 1918
• Main Authors: Shahabi, Arman, Naghili, Behrooz, Ansarin, Khalil, Montazeri, Vahid, Zarghami, Nosratollah
• Format: Journal Article
• Language: English
• Published: Thailand West Asia Organization for Cancer Prevention 01.07.2020
• Subjects: Adult; Aged; Biomarkers, Tumor - genetics; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Case-Control Studies; Female; Follow-Up Studies; Gene Expression Regulation, Neoplastic; Humans; MicroRNAs - genetics; Middle Aged; Prognosis; Survival Rate; Biomarkers; breast cancer; miR-140; Quantitative PCR; miR-196a
• ISSN: 1513-7368; 2476-762X
• DOI: 10.31557/APJCP.2020.21.7.1913

MiR-140 and miR-196a were known to be correlated with cancer diagnosis and prognosis. The current study aimed at the analysis of miR-140 and miR-196a expression patterns and their clinical significance for breast cancer (BC) patients. Differentially expressed miR-140 and miR-196a were examined via quantitative PCR in 110 cases of BC and their adjacent non-tumor (ANT) tissues. The results indicated that miR-140 and miR-196a, respectively, notably decreased and increased expression in BC samples in comparison with ANT (p<0.001). Reduced miR-140 expression was also related to Lymph node metastasis (LNM, P= 0.023) and stage (P = 0.009). Additionally, Receiver Operating Characteristics (ROC) analysis illustrated that miR-140 had a significant diagnostic accuracy for stage and LNM of BC patients. We also discovered a strong negative correlation between miR-196a expression with histological grade (P = 0.038), LNM (P = 0.012) and stage (P = 0.001). Overall, exploring the miR-140 and miR-196a profiles not only can statistically different among BC and ANT samples, but it is also expected to become potential BC biomarkers. .