New Molecular Phylogenetic Evidence Confirms Independent Origin of Coxal Combs in the Families of the 'Cydnoid' Complex (Hemiptera: Heteroptera: Pentatomoidea)

• Published in: Insects (Basel, Switzerland) Vol. 15; no. 10; p. 792
• Main Authors: Lis, Jerzy A, Domagała, Paweł J, Lis, Barbara
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.10.2024; MDPI
• Subjects: Adaptation; Biotechnology; burrower bugs; Cydnidae; Cytochrome; Deoxyribonucleic acid; DNA; DNA sequencing; Ethanol; Gene sequencing; Genes; Hemiptera; Heteroptera; Insects; Mitochondrial DNA; Morphology; Nucleotide sequence; Nucleotide sequencing; Parastrachiidae; Pentatomoidea; Phylogenetics; Phylogeny; rRNA 16S; Sequence analysis; Setae; Soil structure; Species; Thaumastellidae; Thyreocoridae; Poland; United Kingdom; Germany; 16S rDNA; morphology; Parastrachiidae; phylogeny; Cydnidae; Thaumastellidae; burrower bugs; Thyreocoridae
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects15100792

Coxal combs, found only in members of the 'cydnoid' complex (comprising four families: Cydnidae, Parastrachiidae, Thaumastellidae, and Thyreocoridae) within the superfamily Pentatomoidea, have long been regarded as a character confirming their close evolutionary relationship. However, many studies have demonstrated that these four families are phylogenetically distant. Others have been treated as subfamilies of the broadly defined Cydnidae, with the coxal combs as the only character linking them. This is the first study on the origin of coxal combs in species of all families and subfamilies that represent the broadly conceived Cydnidae (69 species in 39 genera). Moreover, this study presents the first 16S rDNA gene sequences providing a basis for such analyses. The analyses included DNA sequences of 62 species in 34 genera of Cydnidae , three species in two genera of Thyreocoridae, two species in two genera of Parastrachiidae, and two species in one genus of Thaumastellidae. The sequence analysis in the family Cydnidae covered 35 species representing 19 genera of the subfamily Cydninae, 16 species in eight genera of the subfamily Sehirinae, five species in two genera of Amnestinae, three species in three genera of Garsauriinae, two species in one genus of Cephalocteinae, and one species of Amaurocorinae. The results of our study demonstrate the independent origin of coxal combs in taxa of the 'cydnoid' complex within the superfamily Pentatomoidea. They confirm the polyphyly not only of the entire 'cydnoid' complex but also of the family Cydnidae itself.