A serum-free defined medium capable of supporting growth of four established human prostatic carcinoma cell lines

This paper describes a serum-free defined medium (Gc) that was initially designed to support growth of the human prostatic carcinoma cell line LNCaP. Our studies indicate that this medium formulation is capable of supporting short-term, long-term, and clonal growth of the LNCaP cell line. Component...

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Bibliographic Details
Published inThe Prostate Vol. 24; no. 5; p. 221
Main Authors Hedlund, T E, Miller, G J
Format Journal Article
LanguageEnglish
Published United States 01.05.1994
Subjects
Adenocarcinoma - pathology
alpha-Fetoproteins - physiology
Cell Division
Clone Cells
Culture Media, Serum-Free
DNA, Neoplasm - analysis
Humans
Insulin - physiology
Karyotyping
Male
Prostatic Neoplasms - pathology
Triiodothyronine - physiology
Tumor Cells, Cultured
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Summary:This paper describes a serum-free defined medium (Gc) that was initially designed to support growth of the human prostatic carcinoma cell line LNCaP. Our studies indicate that this medium formulation is capable of supporting short-term, long-term, and clonal growth of the LNCaP cell line. Component deletion experiments have shown that the three most critical components for LNCaP short-term growth are insulin, triiodothyronine (T3), and fetuin. Additionally, this medium was found to support short-term and clonal growth of three other human prostatic carcinoma cell lines, DU 145, PC-3, and ALVA-31. The availability of such a medium should aid in the distinction of the regulatory factors involved in growth and differentiation of malignant prostatic epithelium.
ISSN:0270-4137
DOI:10.1002/pros.2990240502