The Other Estrogen Receptor in the Plasma Membrane: Implications for the Actions of Environmental Estrogens

Environmental or nutritional estrogenic toxicants are thought to mediate developmental and carcinogenic pathologies. Estrogen receptor (ER) measurements are currently used to predict hormonal responsiveness; therefore all ER subpopulations should be considered. We have been involved in the immunoide...

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Bibliographic Details
Published inEnvironmental health perspectives Vol. 103; no. suppl 7; pp. 41 - 50
Main Authors Watson, Cheryl S., Pappas, Todd C., Gametchu, Bahiru
Format Journal Article
LanguageEnglish
Published United States National Institute of Environmental Health Sciences. National Institutes of Health. Department of Health, Education and Welfare 01.10.1995
Subjects
Cell lines
Cell Membrane - metabolism
Cell membranes
Environmental Pollutants - metabolism
Estrogens
Estrogens - metabolism
Granulosa cells
Lymphocytes
Membrane Proteins - physiology
Neurons
Nontraditional Sites of Estrogen Action
P branes
Receptors
Receptors, Estrogen - metabolism
Steroid receptors
Steroids
Tumor Cells, Cultured
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Summary:Environmental or nutritional estrogenic toxicants are thought to mediate developmental and carcinogenic pathologies. Estrogen receptor (ER) measurements are currently used to predict hormonal responsiveness; therefore all ER subpopulations should be considered. We have been involved in the immunoidentification and characterization of membrane steroid receptors in several systems and have recently shown that binding of estradiol ( E2) to a subpopulation of ERs (mER) residing in the plasma membrane of GH3pituitary tumor cells mediates the rapid release of prolactin (PRL). Here we review these findings and present other important characterizations of these receptors such as trypsin and serum susceptibility, movement in the membrane, confocal localization to the membrane, binding to and function of impeded ligands, and immunoseparation of cells bearing mER. We plan to use this system as a model for both the physiological and pathological nongenomic effects of estrogens and estrogenic xenobiotics. Specifically, it should be useful as an in vitro assay system for the ability of estrogenic xenobiotics to cause rapid PRL release as an example of nongenomic estrogen effects.
ISSN:0091-6765
1552-9924
DOI:10.1289/ehp.95103s741