Wavelength-Resolved Flow Cytometer Under a Dark-Field Illumination Configuration

• Published in: IEEE sensors journal Vol. 11; no. 11; pp. 2845 - 2851
• Main Authors: Shi-Wei Lin, Pin-Yao Wang, Chen, A., Chih-Han Chang, Che-Hsin Lin
• Format: Journal Article
• Language: English
• Published: New York IEEE 01.11.2011; The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
• Subjects: Bulbs; Channels; Chips; Classification; Counting; dark-field illumination; diascopic configuration; Fluorescence; Illumination; Indexes; Light sources; Lighting; Microflow cytometry; Microfluidics; multispectral detection; Optical filters; Optical scattering; Spectra
• ISSN: 1530-437X; 1558-1748
• DOI: 10.1109/JSEN.2011.2170061

This paper presents a novel yet high-performance diascopic illumination configuration for simultaneously detecting cells of different sizes and different fluorescence labeling in a microfluidic chip. An objective-type dark-field condenser equipped with a low-cost tungsten bulb light source with continuous wavelengths (400 to 900 nm) and an UV-Vis-NIR spectrometer is used for detecting the multispectral signals from various particles and cells. With this approach, continuous cell counting and spectra analyzing are realized in a single channel without using any spatial filter and delicate optical detectors. Results show that the developed system is capable of identifying different labeled particle and cell samples by extracting the information side-scatter, absorption, and fluorescence from the information-rich spectra. This proposed system provides an efficient multicolor detection for identification and classification of a microflow cytometer chip.