Bioprospecting of cyanobacterium in Chilean coastal desert, Geitlerinema sp. molecular identification and pressurized liquid extraction of bioactive compounds

• Published in: Food and bioproducts processing Vol. 128; pp. 227 - 239
• Main Authors: Ruiz-Domínguez, M.C., Fuentes, J.L., Mendiola, J.A., Cerezal-Mezquita, P., Morales, J., Vílchez, C., Ibáñez, E.
• Format: Journal Article
• Language: English
• Published: Rugby Elsevier B.V 01.07.2021; Elsevier Science Ltd
• Subjects: Algae; Bioactive compounds; Biological activity; Bioprospecting; Biorefineries; Cyanobacteria; Cyanobacterium; Deserts; Design factors; Ethanol; Factorial design; Fatty acids; Geitlerinema; High temperature; Industrial applications; Limonene; Lipids; Low temperature; Molecular identification; Palmitic acid; Phycobiliproteins; Phycocyanin; Phylogeny; Pressurized liquid extraction (PLE); Response surface methodology; Solvent extraction; Solvents; Yield; Lipids; Cyanobacterium; Phycobiliproteins; Pressurized liquid extraction (PLE); Fatty acids; Molecular identification
• ISSN: 0960-3085; 1744-3571
• DOI: 10.1016/j.fbp.2021.06.001

[Display omitted] •Isolated cyanobacterium from the Chilean coastal desert was genus Geitlerinema.•Geitlerinema sp. showed to be a rich source of proteins and phycobiliproteins.•Extraction yield increased with water and high temperature under PLE.•Lipids recovery improved under polar extractants as the temperature increased. This study presents a new cyanobacterium of the genus Geitlerinema sp. isolated from the Chilean coastal desert as a rich source of bioactive compounds. Phylogenetic tree is shown along with a broad biochemical characterization using conventional and pressurized liquid extraction. Variables as extraction yield, phycobiliproteins, lipids and methyl palmitate (C16:0) were studied by means of two experimental designs based on a response surface methodology (RSM) employing two factorial design 32. Factors as temperature (20–200 °C) and percentage of solvents were selected resulting in Factorial Design A with 0–100% (v/v) of ethanol–water and Factorial Design B with 0–100 (v/v) of ethanol–limonene solvents. The main bioactive compounds were total proteins (∼52% wt), phycobiliproteins (especially C-phycocyanin) and methyl palmitate (C16:0). Data obtained showed that water and ethanol:water (1:1, v/v) improved the extraction yield and phycobiliproteins recovery. As for ethanol:limonene extracts, they were better for lipids and methyl palmitate extractions. In general, high temperatures only improved the extraction yield whereas low temperatures favor the extraction of phycobiliproteins. Therefore, results indicated that Geitlerinema sp. could be one of the several cyanobacteria genera that display functional properties for nutritional, pharmaceutical or industrial use as sustainable and cost-effective bioactive compounds in algal biorefinery model.