Effect of osmotic pressure on cell growth and production of ginseng saponin and polysaccharide in suspension cultures of Panax notoginseng

The effects of initial osmotic pressure (IOP) on the production of ginseng polysaccharide and ginseng saponin were studied in suspension cultures of Panax notoginseng cells. At higher IOP, the specific saponin production and intracellular carbohydrate storage were increased, while the plant cell vol...

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Bibliographic Details
Published inBiotechnology letters Vol. 17; no. 12; pp. 1347 - 1350
Main Authors Zhang, Yi-Heng, Zhong, Jian-Jiang, Yu, Jun-Tang
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.12.1995
Subjects
Biological and medical sciences
Biotechnology
Establishment of new cell lines, improvement of cultural methods, mass culture
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Plant cells and fungal cells
Culture medium
Cell proliferation
Cell culture
Vegetals
Growth rate
Environmental factor
Saponin
Suspension culture
Dicotyledones
Angiospermae
Production
Spermatophyta
Carbohydrate
Kinetics
Intracellular
Osmotic pressure
Polysaccharide
Araliaceae
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Summary:The effects of initial osmotic pressure (IOP) on the production of ginseng polysaccharide and ginseng saponin were studied in suspension cultures of Panax notoginseng cells. At higher IOP, the specific saponin production and intracellular carbohydrate storage were increased, while the plant cell volume, the consumption rates of major medium components and the specific cell growth rate were decreased. The specific production of polysaccharide was reduced with an increase of IOP from 4.45 to 5.18 atm, and levelled off at an even higher IOP.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-5492
1573-6776
DOI:10.1007/BF00189224