Study on the supramolecular interaction between β-cyclodextrin and gemfibrozil by flow injection spectrofluorimetry and its analytical application

• Published in: Analytica chimica acta Vol. 516; no. 1; pp. 221 - 227
• Main Authors: Tang, Bo, Jia, Baoxiu, Cui, Guanwei, Ding, Yi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 19.07.2004; Elsevier
• Subjects: Analysis; Analytical chemistry; Biological and medical sciences; Chemistry; Exact sciences and technology; Flow injection; Gemfibrozil; General pharmacology; Medical sciences; Pharmacology. Drug treatments; Spectrofluorimetry; Spectrometric and optical methods; Supramolecular interaction; β-Cyclodextrin; β-Cyclodextrin; Spectrofluorimetry; Gemfibrozil; Supramolecular interaction; Flow injection; Trace analysis; Complexation; Association constant; Capsule; Fluorescence spectrometry; Cyclodextrin; Emission spectrometry; Detection limit; Serum; Aqueous solution; Sampling
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2004.03.065

The supramolecular interaction of gemfibrozil and β-cyclodextrin has been studied by flow injection spectrofluorimetry. The results show that β-CD reacts with gemfibrozil to form a 1:1 complex with an apparent association constant of 7.57×10 2 L/mol. Based on the enhancement of the fluorescence intensity of gemfibrozil, a flow injection spectrofluorimetric method for the determination of gemfibrozil in bulk aqueous solution in the presence of β-CD was developed. The linear range was 0.032–70.00 μg/mL. The detection limit was 9.6 ng/mL with a sampling rate of 80 h −1. There was no interference from the excipients normally used in tablet formulations and the serum compositions. The proposed method was successfully applied to the determination of gemfibrozil in capsules and serum.