A new conductometric biosensor based on horseradish peroxidase immobilized on chitosan and chitosan/gold nanoparticle films
Abstract A new conductometric biosensor was developed and characterized; the biosensor was based on horseradish peroxidase that was deposited in chitosan and chitosan/AuNPs films. The biosensors were characterized by scanning electron microscopy and current-voltage curves. Current-voltage curves in...
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| Published in | Journal of polymer engineering Vol. 34; no. 7; pp. 633 - 638 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
01.09.2014
|
| Online Access | Get full text |
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| Summary: | Abstract
A new conductometric biosensor was developed and characterized; the biosensor was based on horseradish peroxidase that was deposited in chitosan and chitosan/AuNPs films. The biosensors were characterized by scanning electron microscopy and current-voltage curves. Current-voltage curves in biosensors showed that the electrical conductivity and bistability in biosensors can be modulated by horseradish peroxidase. Horseradish peroxidase catalyzed the reduction of H
2
O
2
to H
2
O with the oxidation of the prosthetic group (Fe
3+
) in the enzyme to Fe
4+
=O. Conductometric signal in the biosensors increased with the gradual increase of H
2
O
2
concentration, and it was due to the H
2
O
2
reduction. Linear hydrogen peroxide detection was observed for a concentration between 0 and 15 m
m
. The results proved that these biosensors could have promising industrial applications, due to its rapid and sensitive H
2
O
2
detection. |
|---|---|
| ISSN: | 0334-6447 2191-0340 |
| DOI: | 10.1515/polyeng-2014-0072 |