A detective monoclonal antibody recognized conserved epitope of hemagglutinin from H5N1 influenza virus

Monoclonal antibodies are needed for the development of effective, accurate and sensitive immunoassays against the H5N1 avian influenza. Here we report the generation of monoclonal antibodies targeting Escherichia coli expressed hemagglutinin of HA1 domain from H5N1 influenza virus. The monoclonal a...

Full description

Saved in:
Bibliographic Details
Published inComptes rendus. Chimie Vol. 14; no. 9; pp. 826 - 830
Main Authors Zhuang, Xiaoxiao, Sun, Yizhuo, Ling, Zhiyang, Dong, Qingxi, Zhu, Jingyan, Tang, Linna, Zhou, Yanyan, Ji, Yongyong, Tian, Lin, Lin, Guomei, Yan, Lingchen, Huang, Chaofeng, Chen, Ze, Bian, Chao, Sun, Bing
Format Journal Article Conference Proceeding
LanguageEnglish
Published Paris Elsevier SAS 01.09.2011
Elsevier
Subjects
Antibodies
Antigens
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Immunoassays
Immunology
Microbiology
Proteins
Virology
Proteins
Antibodies
Antigens
Immunology
Immunoassays
Virus
Influenzavirus A(H5N1)
Influenzavirus A
Antigenic determinant
Orthomyxoviridae
Monoclonal antibody
Online AccessGet full text

Cover

More Information
Summary:Monoclonal antibodies are needed for the development of effective, accurate and sensitive immunoassays against the H5N1 avian influenza. Here we report the generation of monoclonal antibodies targeting Escherichia coli expressed hemagglutinin of HA1 domain from H5N1 influenza virus. The monoclonal antibodies can be used to establish a sandwich ELISA assay that is able to detect HA from H5 subtype virus lysate but not from H9 subtype. A subtype-conserved new epitope was also identified which is located between the amino acid residues N80 to A99. This work would be helpful for the development of a diagnosis kit for the H5N1 avian influenza.
ISSN:1631-0748
1878-1543
1878-1543
DOI:10.1016/j.crci.2011.04.001